| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Finn, Sarah E (10)
|
OHBASH |
36.92 |
34.22 |
-2.70 |
| 2 |
Mulkey, Lyla C (9)
|
OHPCY |
41.25 |
37.82 |
-3.43 |
| 3 |
Gould, Addison M (9)
|
OHPCY |
38.02 |
37.96 |
-0.06 |
| 4 |
Fox, Chloe G (9)
|
OHPCY |
|
39.27 |
- |
| 5 |
Hornbaker, Drury L (9)
|
OHPCY |
43.00 |
40.15 |
-2.85 |
| 6 |
Johannesmeyer, Rachel M (10)
|
OHPCY |
|
40.44 |
- |
| 7 |
McCormick, Emma K (7)
|
OHPCY |
|
40.81 |
- |
| 8 |
Schutte, Erin L (10)
|
OHBASH |
49.98 |
41.30 |
-8.68 |
| 9 |
Murphy, Rosemary C (9)
|
OHPCY |
45.13 |
41.61 |
-3.52 |
| 10 |
Detweiler, Liara G (10)
|
OHBASH |
40.58 |
43.56 |
2.98 |
| 11 |
Li, Xinran (8)
|
OHBASH |
|
46.23 |
- |
| 12 |
Hile, Caroline A (10)
|
OHPCY |
53.13 |
48.53 |
-4.60 |
| 13 |
Larson, Maya J (10)
|
OHPCY |
53.09 |
48.61 |
-4.48 |
| 14 |
Nobbe, Ava M (10)
|
OHPCY |
1:06.59 |
49.80 |
-16.79 |
| 15 |
Hammer, Annabelle L (7)
|
OHPCY |
|
51.27 |
- |
| 16 |
Khadka, Sana S (9)
|
OHBASH |
55.25 |
52.24 |
-3.01 |
| 17 |
Martinez-Rentas, Isabela S (10)
|
OHBASH |
|
54.88 |
- |
| 18 |
Belachew, Hulumelkam T (10)
|
OHPCY |
|
55.70 |
- |
| 19 |
Dye, Charlotte M (6)
|
OHPCY |
1:15.79 |
57.12 |
-18.67 |
| 20 |
White, Leah R (8)
|
OHPCY |
1:07.95 |
57.47 |
-10.48 |
| 21 |
Kolagi, Stuti R (9)
|
OHBASH |
59.17 |
58.95 |
-0.22 |
| 22 |
Gribbell, Alice K (9)
|
OHPCY |
1:23.25 |
1:01.76 |
-21.49 |
| 23 |
Chrapliwy, Kate E (8)
|
OHPCY |
|
1:05.39 |
- |
| 24 |
Herbst, Arianna R (6)
|
OHPCY |
|
1:07.78 |
- |
| 25 |
Azpiroz Galante, Nico (8)
|
OHBASH |
|
1:08.32 |
- |
| 26 |
Mohan, Anushka (8)
|
OHBASH |
|
1:08.36 |
- |
| 27 |
Belachew, Wubet T (10)
|
OHPCY |
|
1:12.30 |
- |
| 28 |
Frey, Etta L (8)
|
OHPCY |
|
1:32.62 |
- |