| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rudowski, Faith E (14)
|
OHCY |
52.13 |
56.31 |
4.18 |
| 2 |
Spencer, Olivia (13)
|
OHPCY |
58.75 |
1:00.16 |
1.41 |
| 3 |
Lewis, Anna V (14)
|
OHBASH |
58.54 |
1:01.13 |
2.59 |
| 4 |
Smith, Annalise M (14)
|
OHBASH |
57.89 |
1:01.89 |
4.00 |
| 5 |
Feichtner, Jane D (14)
|
OHPCY |
1:02.77 |
1:03.19 |
0.42 |
| 6 |
Schweikert, Melanie G (14)
|
OHCY |
1:04.95 |
1:03.29 |
-1.66 |
| 7 |
Garza, Ella D (14)
|
OHPCY |
|
1:04.21 |
- |
| 8 |
Perry, Gabby O (14)
|
OHCY |
1:03.96 |
1:05.12 |
1.16 |
| 9 |
Chu, Kylene R (14)
|
OHPCY |
1:02.25 |
1:05.15 |
2.90 |
| 9 |
Eldridge, Yamileth L (13)
|
OHPCY |
1:03.48 |
1:05.15 |
1.67 |
| 11 |
Brubaker, Josephine E (14)
|
OHPCY |
1:04.42 |
1:05.77 |
1.35 |
| 12 |
Robles, Eva (14)
|
OHPCY |
1:07.59 |
1:06.53 |
-1.06 |
| 13 |
Gamble, Hazelle D (13)
|
OHPCY |
1:11.97 |
1:07.69 |
-4.28 |
| 14 |
Castenson, Zoe A (13)
|
OHBASH |
1:09.26 |
1:08.09 |
-1.17 |
| 15 |
Jutze, Molly E (14)
|
OHBASH |
|
1:09.28 |
- |
| 16 |
Gliatti, Isabella J (14)
|
OHCY |
1:05.81 |
1:13.00 |
7.19 |
| 17 |
Kirby, MacKenzie I (13)
|
OHBASH |
|
1:25.67 |
- |
|
Harker, Charlie A (14)
|
OHCY |
58.60 |
NS |
- |
|
Vander Veen, Kelly J (14)
|
OHPCY |
1:37.77 |
NS |
- |