| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Matern, Sofia E (14)
|
OHABLY |
2:24.01 |
2:29.56 |
5.55 |
| 2 |
Mitchell, Lily M (13)
|
OHABLY |
2:31.11 |
2:32.64 |
1.53 |
| 3 |
Pribonic, Isabella E (13)
|
OHPHST |
2:38.56 |
2:39.99 |
1.43 |
| 4 |
Luckett, Kate J (13)
|
OHCY |
2:47.69 |
2:42.59 |
-5.10 |
| 5 |
Zimpfer, Lola W (13)
|
OHOSSC |
2:38.27 |
2:42.63 |
4.36 |
| 6 |
Brink, Audrey E (13)
|
OHUSA |
2:41.34 |
2:45.42 |
4.08 |
| 7 |
Scott, Alivia M (14)
|
OHCM |
2:50.03 |
2:46.04 |
-3.99 |
| 8 |
Minamyer, Erinn E (14)
|
OHUSA |
2:52.82 |
2:46.19 |
-6.63 |
| 9 |
Scharpf, Alexandra L (13)
|
LESTRS |
2:44.35 |
2:47.10 |
2.75 |
| 10 |
Peltcs, Avery M (14)
|
OHUSA |
2:42.60 |
2:47.36 |
4.76 |
| 11 |
Elliott, Olivia K (14)
|
OHWOR |
2:45.83 |
2:47.64 |
1.81 |
| 12 |
Tropp, Emily E (13)
|
OHSPY |
2:46.60 |
2:48.59 |
1.99 |
| 13 |
Morrison, Sarah (13)
|
OHCFY |
2:46.06 |
2:49.73 |
3.67 |
| 14 |
Muzychak, Ella M (14)
|
LELSSC |
2:46.60 |
2:52.37 |
5.77 |
| 15 |
Polyakov, Sophia S (13)
|
LESTRS |
2:51.79 |
2:52.48 |
0.69 |
| 16 |
McCusker, Adeline V (14)
|
OHOSSC |
2:54.11 |
2:54.78 |
0.67 |
| 17 |
Rosko, Giovanna G (14)
|
LESTRS |
2:58.15 |
2:56.44 |
-1.71 |
| 18 |
Gammon, Annie M (13)
|
OHOHYA |
2:53.55 |
2:56.57 |
3.02 |
| 19 |
Clapp, Caden E (13)
|
OHCY |
2:57.42 |
3:08.04 |
10.62 |