| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Finn, Sarah E (10)
|
OHBASH |
48.72 |
48.55 |
-0.17 |
| 2 |
Wallbrown, Katherine G (10)
|
OHBASH |
52.63 |
50.19 |
-2.44 |
| 3 |
Fox, Chloe G (9)
|
OHPCY |
|
53.13 |
- |
| 4 |
Murphy, Rosemary C (9)
|
OHPCY |
1:01.42 |
53.99 |
-7.43 |
| 5 |
Mallow, Kristen M (10)
|
OHBASH |
|
54.73 |
- |
| 6 |
Schutte, Erin L (10)
|
OHBASH |
57.10 |
57.32 |
0.22 |
| 7 |
Hornbaker, Drury L (9)
|
OHPCY |
|
57.81 |
- |
| 8 |
Li, Xinran (8)
|
OHBASH |
|
57.88 |
- |
| 9 |
Nobbe, Ava M (10)
|
OHPCY |
|
1:00.15 |
- |
| 10 |
Hile, Caroline A (10)
|
OHPCY |
|
1:04.39 |
- |
| 11 |
Kolagi, Stuti R (9)
|
OHBASH |
|
1:05.74 |
- |
| 12 |
Khadka, Sana S (9)
|
OHBASH |
|
1:05.77 |
- |
| 13 |
Martinez-Rentas, Isabela S (10)
|
OHBASH |
|
1:08.33 |
- |
|
Azpiroz Galante, Nico (8)
|
OHBASH |
|
DQ |
- |
|
Larson, Maya J (10)
|
OHPCY |
|
DQ |
- |