| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gildea, Parker M (8)
|
LELSSC |
43.88 |
40.20 |
-3.68 |
| 2 |
Sagle, Tyler J (7)
|
OHOSSC |
45.38 |
41.11 |
-4.27 |
| 3 |
Xie, Ethan W (8)
|
OHCM |
45.45 |
44.30 |
-1.15 |
| 4 |
Brewer, Nathan E (8)
|
LELSSC |
44.86 |
44.51 |
-0.35 |
| 5 |
Booms, Benjamin E (8)
|
OHCM |
47.11 |
45.65 |
-1.46 |
| 6 |
Moore, Matthew S (8)
|
OHOSSC |
47.15 |
46.14 |
-1.01 |
| 7 |
Powers, Liam J (8)
|
OHUSA |
51.48 |
49.66 |
-1.82 |
| 8 |
Metzger, Parker T (8)
|
OHCM |
54.80 |
51.23 |
-3.57 |
| 9 |
Wilson, Bradley M (7)
|
LELSSC |
53.69 |
51.28 |
-2.41 |
| 10 |
Razzano, Dominic T (7)
|
OHCM |
52.21 |
53.11 |
0.90 |
| 11 |
Beischel, Emerson P (7)
|
OHCM |
52.29 |
53.76 |
1.47 |
| 12 |
Gilpin, Gio B (7)
|
OHCM |
1:02.98 |
54.13 |
-8.85 |
| 13 |
Beischel, Graham T (7)
|
OHCM |
57.60 |
55.55 |
-2.05 |
| 14 |
Stone, Kellen E (8)
|
OHCM |
58.90 |
55.84 |
-3.06 |
| 15 |
Brewer, Zachary I (7)
|
LELSSC |
1:01.76 |
56.57 |
-5.19 |
| 16 |
Oswiecinski, Ryan M (7)
|
OHDCST |
1:04.52 |
56.96 |
-7.56 |
| 17 |
Nadur, Noah M (6)
|
OHDCST |
1:01.78 |
59.49 |
-2.29 |
| 18 |
Sprengard, Brendan C (8)
|
OHCM |
57.85 |
59.50 |
1.65 |
| 19 |
Obid, Jacob R (6)
|
LELSSC |
56.31 |
59.93 |
3.62 |
| 20 |
Subramanian, Abhinav Ram (8)
|
LESWIM |
1:09.14 |
1:01.45 |
-7.69 |
| 21 |
Knepfle, John H (7)
|
OHCM |
1:27.54 |
1:31.80 |
4.26 |
|
Tan, Noah T (5)
|
OHCM |
1:22.87 |
DQ |
- |
|
Pennington, Richard E (8)
|
OHWOR |
47.81 |
NS |
- |