| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schneider, Victoria C (13)
|
OHLAKY |
2:48.09 |
2:46.22 |
-1.87 |
| 2 |
Dirst, Julianne R (14)
|
OHRCDB |
2:52.58 |
2:50.49 |
-2.09 |
| 3 |
Williams, Makenna G (14)
|
OHABLY |
2:55.37 |
2:54.73 |
-0.64 |
| 4 |
Bhatti, Angelica (14)
|
OHLAKY |
2:57.50 |
2:55.89 |
-1.61 |
| 5 |
Koetter, Elizabeth S (14)
|
OHBASH |
3:03.00 |
2:58.32 |
-4.68 |
| 6 |
Phillips-Culbertson, Ariana (14)
|
OHLAKY |
2:57.31 |
2:59.10 |
1.79 |
| 7 |
Crowe, Kayleen A (14)
|
OHLAKY |
3:03.34 |
2:59.71 |
-3.63 |
| 8 |
Ingram, Katherine E (13)
|
OHCY |
3:00.13 |
3:00.00 |
-0.13 |
| 9 |
Jutze, Molly E (14)
|
OHBASH |
3:03.54 |
3:01.03 |
-2.51 |
| 10 |
Cherry, Hannah R (14)
|
OHCY |
3:05.61 |
3:08.58 |
2.97 |