| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kalac, Rory J (17)
|
GUBATS |
28.15 |
29.13 |
0.98 |
| 2 |
Walsh, Paige J (16)
|
GUPACK |
29.47 |
29.62 |
0.15 |
| 3 |
Guice, Richelle J (16)
|
GUUN-B |
31.01 |
30.23 |
-0.78 |
| 4 |
Molinaro, Mikaela G (15)
|
GUPACK |
29.51 |
30.44 |
0.93 |
| 5 |
Gottfried, Charlotte A (16)
|
GUPACK |
30.33 |
31.02 |
0.69 |
| 6 |
Smith, Megan G (15)
|
GUPACK |
30.91 |
31.13 |
0.22 |
| 7 |
Revelas, Lily T (16)
|
GUPACK |
28.83 |
31.27 |
2.44 |
| 8 |
Plunkett, Abigail R (16)
|
GUPACK |
30.66 |
31.96 |
1.30 |
| 9 |
O'Sullivan, Mary K (15)
|
GUPACK |
30.99 |
32.09 |
1.10 |
| 10 |
Roth, Kayla M (15)
|
GUPACK |
31.23 |
32.21 |
0.98 |
| 11 |
Rozelle, Laura M (15)
|
GUBATS |
|
32.32 |
- |
| 12 |
Noonan, Miranda B (15)
|
GUBATS |
32.52 |
32.34 |
-0.18 |
| 13 |
Torres, Celia G (17)
|
GUPACE |
31.41 |
32.35 |
0.94 |
| 14 |
Crawford, Cameron R (15)
|
GUPACK |
32.11 |
33.23 |
1.12 |
| 15 |
Wasik, Morgan L (16)
|
GUPACK |
33.44 |
33.30 |
-0.14 |
| 16 |
Lindsey, Kyla J (15)
|
GUPACE |
34.77 |
34.57 |
-0.20 |
| 17 |
Jensen-Morgan, Olivia Y (16)
|
GUBATS |
35.28 |
35.60 |
0.32 |
| 18 |
Sale, Hannah B (16)
|
GUPACK |
|
1:03.75 |
- |