| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wang, Jason H (11)
|
GUKATY |
38.77 |
35.91 |
-2.86 |
| 2 |
Spencer, Oliver A (12)
|
GUUN-P |
37.83 |
35.92 |
-1.91 |
| 3 |
Serpa, Arnaldo G (11)
|
GUESCA |
|
36.34 |
- |
| 4 |
Picen, Gianluca J (12)
|
GUESCA |
38.39 |
37.09 |
-1.30 |
| 5 |
Liu, Geoffrey * (11)
|
GUPEAK |
38.31 |
37.48 |
-0.83 |
| 6 |
Nguyen, Anderson K (11)
|
GUESCA |
41.51 |
39.61 |
-1.90 |
| 7 |
LaBrose, Luke A (11)
|
GUKATY |
43.90 |
39.79 |
-4.11 |
| 8 |
Pan, Eric (11)
|
GUKATY |
38.85 |
40.42 |
1.57 |
| 9 |
Tran, Eric H (12)
|
GUESCA |
36.39 |
41.63 |
5.24 |
| 10 |
Hertenberger, Collin W (12)
|
GUPACK |
|
43.08 |
- |
| 11 |
Rojas, Alejandro N (12)
|
GUKATY |
38.28 |
44.05 |
5.77 |
| 12 |
Lucio III, Edelmiro (12)
|
GUKATY |
38.70 |
44.56 |
5.86 |
| 13 |
Hertenberger, Christopher D (12)
|
GUPACK |
|
44.59 |
- |
| 14 |
Heiman, Luke M (11)
|
GUPACK |
45.46 |
45.43 |
-0.03 |
| 15 |
Doan, Dawson A (11)
|
GULSST |
49.00 |
46.19 |
-2.81 |
| 16 |
Cook, Luke W (12)
|
GUPEAK |
46.96 |
46.37 |
-0.59 |