| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mintz, Dominic S (12)
|
NICLSC |
31.48 |
30.77 |
-0.71 |
| 2 |
Adams, Marshall B (12)
|
NITWST |
32.79 |
32.32 |
-0.47 |
| 3 |
Norvilitis, Matthew J (12)
|
NITWST |
34.69 |
33.49 |
-1.20 |
| 4 |
Wichhart, Benjamin A (11)
|
NIWWSC |
38.15 |
34.64 |
-3.51 |
| 5 |
Steffens, Michael H (12)
|
NITTAC |
40.21 |
35.55 |
-4.66 |
| 6 |
Schucker, Brian M (12)
|
NIWWSC |
37.71 |
36.03 |
-1.68 |
| 7 |
Gatti, Jake D (11)
|
NIRAYS |
40.03 |
38.07 |
-1.96 |
| 8 |
LaMoy, Hunter P (11)
|
NIRAYS |
39.94 |
39.26 |
-0.68 |
| 9 |
Nietopski, Wyatt M (12)
|
NIRAYS |
39.91 |
40.00 |
0.09 |
| 10 |
Bartolotti, Harry D (12)
|
NITWST |
39.50 |
40.97 |
1.47 |