| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Marchese, Nicole (14)
|
NICLSC |
2:28.10 |
2:29.78 |
1.68 |
| 2 |
Forcucci, Angela (13)
|
NITWST |
2:38.06 |
2:34.54 |
-3.52 |
| 3 |
Castonguay, Laura J (13)
|
NITWST |
2:42.48 |
2:34.65 |
-7.83 |
| 4 |
Maslanka, Julia E (14)
|
NIFALC |
2:37.76 |
2:37.26 |
-0.50 |
| 5 |
Dallas, Elizabeth J (14)
|
NIFALC |
2:57.48 |
2:41.90 |
-15.58 |
| 6 |
Mueller, Maegan G (13)
|
NIWWSC |
2:51.90 |
2:42.42 |
-9.48 |
| 7 |
Palmeri, Olivia M (13)
|
NICLSC |
2:43.56 |
2:42.56 |
-1.00 |
| 8 |
Miterko, Hannah C (13)
|
NITWST |
2:51.73 |
2:46.89 |
-4.84 |
| 9 |
Johnson, Emma E (13)
|
NITWST |
2:52.41 |
2:47.50 |
-4.91 |
| 10 |
Viscuso, Meadow L (13)
|
NICLSC |
2:49.96 |
2:48.17 |
-1.79 |
| 11 |
Kwiatkowski, Ella C (13)
|
NICLSC |
2:53.74 |
2:51.44 |
-2.30 |
| 12 |
Baker, Kelly E (14)
|
NICLSC |
3:05.25 |
2:59.49 |
-5.76 |
| 13 |
Steffens, Carolynn S (14)
|
NITTAC |
2:53.03 |
3:01.76 |
8.73 |
| 14 |
Lane, Morgan E (13)
|
NITWST |
3:06.60 |
3:08.25 |
1.65 |
| 15 |
Hogle, Brooke E (13)
|
NITWST |
3:16.96 |
3:14.88 |
-2.08 |
| 16 |
Heinrich, Chloe M (13)
|
NITWST |
3:20.44 |
3:16.74 |
-3.70 |
| 17 |
Redeye, Elsie I (13)
|
NISAAC |
|
3:17.93 |
- |
| 18 |
Regan, Abby S (13)
|
NITWST |
3:27.06 |
3:22.09 |
-4.97 |
| 19 |
Santangelo, Madison R (13)
|
NITWST |
3:18.61 |
3:22.72 |
4.11 |
| 20 |
Hellwig, Ashleigh R (13)
|
NIHHSC |
|
3:44.18 |
- |
|
Clemens, Megan E (14)
|
NITTAC |
2:49.45 |
DQ |
- |
|
Towne, Julia R (13)
|
NICLSC |
2:48.39 |
NS |
- |