| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Forcucci, Angela (14)
|
NITWST |
2:34.54 |
2:26.86 |
-7.68 |
| 2 |
Costich, Charlotte G (13)
|
NICLSC |
2:27.81 |
2:31.05 |
3.24 |
| 3 |
Gentile, Juliana (14)
|
NIVELO |
2:42.61 |
2:36.21 |
-6.40 |
| 4 |
Clouser, Kristiana I (13)
|
NIBCSR |
2:42.47 |
2:47.36 |
4.89 |
| 5 |
Higley, Kayla P (14)
|
NIRAYS |
2:55.18 |
2:54.87 |
-0.31 |
| 6 |
Kubiak, Kelly A (13)
|
NITWST |
2:46.54 |
2:55.49 |
8.95 |
| 7 |
Ground, Heather A (14)
|
NISAAC |
3:03.58 |
2:55.60 |
-7.98 |
| 8 |
Langlotz, Olivia M (13)
|
NICLSC |
3:05.91 |
2:56.19 |
-9.72 |
| 9 |
Xu, Emily (14)
|
NIVELO |
2:59.72 |
3:01.28 |
1.56 |
| 10 |
Judy, Paris M (13)
|
NITWST |
3:07.13 |
3:04.60 |
-2.53 |
| 11 |
Barry, Caitlin E (13)
|
NITWST |
3:18.37 |
3:07.15 |
-11.22 |
| 12 |
Hogle, Brooke E (14)
|
NITWST |
3:04.77 |
3:08.28 |
3.51 |
| 13 |
Cameron, Nzingha A (13)
|
NIBCSR |
3:31.84 |
3:26.44 |
-5.40 |
|
Canestaro, Annabelle S (13)
|
NIVELO |
3:08.38 |
NS |
- |