| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Boeman, Joris (12)
|
OHLCWE |
27.46 |
27.48 |
0.02 |
| 2 |
Heck, Andrew (12)
|
DYP |
29.57 |
29.53 |
-0.04 |
| 3 |
Mcintyre, Anthony P (12)
|
LERYD |
32.67 |
30.89 |
-1.78 |
| 4 |
Calvert, Matthew (12)
|
NCY |
32.76 |
32.65 |
-0.11 |
| 5 |
Buchholz, Michael A (12)
|
LEYWRC |
33.01 |
33.38 |
0.37 |
| 6 |
Nguyen, Kenny (11)
|
TUSC |
33.90 |
33.59 |
-0.31 |
| 7 |
Udell, Cooper X (12)
|
LEYWRC |
34.04 |
34.00 |
-0.04 |
| 8 |
Gordon, Ethan B (11)
|
LECFYN |
35.06 |
34.02 |
-1.04 |
| 9 |
Heller, Nick (12)
|
WAD |
34.44 |
34.30 |
-0.14 |
| 10 |
Kacsala, Jonathan (11)
|
LCE |
34.43 |
35.44 |
1.01 |
| 11 |
Craig, Benjamin (11)
|
OHAYF |
35.46 |
35.72 |
0.26 |
| 12 |
Webster, Adam (12)
|
OHLCCY |
36.16 |
36.25 |
0.09 |
| 13 |
Ellenwood, JJ (11)
|
LEAYAA |
37.73 |
36.43 |
-1.30 |
| 14 |
Iskra, Dominic J (11)
|
LEGO |
35.88 |
36.61 |
0.73 |
| 15 |
Marina, Liviu I (12)
|
LERYD |
36.33 |
37.11 |
0.78 |
| 16 |
Edwardson, Nathaniel D (12)
|
LECFYN |
40.42 |
39.78 |
-0.64 |
| 17 |
Lynch, Brogann (11)
|
NCY |
39.97 |
41.40 |
1.43 |
| 18 |
Rini, Alex (12)
|
LEVFYT |
41.12 |
41.45 |
0.33 |
| 19 |
Hofstetter, Luke J (11)
|
OHOYST |
49.36 |
42.68 |
-6.68 |
| 20 |
Hicks, Nathaniel (11)
|
LEAYAA |
42.66 |
43.18 |
0.52 |
| 21 |
Debevec, Ethan (12)
|
LEGO |
45.05 |
43.38 |
-1.67 |
| 22 |
Betscakos, Joaquin N (12)
|
LEGYB |
45.00 |
44.14 |
-0.86 |
| 23 |
Yin, Frank * (11)
|
LERYD |
45.34 |
44.34 |
-1.00 |
| 24 |
Tsagares, Jacob (11)
|
LEYYN |
48.22 |
46.81 |
-1.41 |
| 25 |
Gerics, Marcus (11)
|
LCE |
52.20 |
47.83 |
-4.37 |
|
Huang, Timothy (11)
|
OHLCCY |
40.17 |
DQ |
- |
|
Tate, Ralph (11)
|
LEGYB |
53.95 |
DQ |
- |
|
Senol, Logan (12)
|
OHLCWE |
53.50 |
DQ |
- |
|
Turney, Miles R (11)
|
LECFYN |
1:00.62 |
DQ |
- |