| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smith, Samantha M (14)
|
LEGO |
5:36.07 |
5:35.70 |
-0.37 |
| 2 |
Chelf, Gretchen K (13)
|
LEYWRC |
6:05.46 |
5:45.76 |
-19.70 |
| 3 |
Fernandez, Suzanna R (13)
|
LERYD |
5:56.58 |
5:52.83 |
-3.75 |
| 4 |
Kurtzweil, Bridget J (14)
|
LEVFYT |
5:55.78 |
5:52.92 |
-2.86 |
| 5 |
Angerstien, Kali E (13)
|
LEGYB |
6:10.83 |
5:59.43 |
-11.40 |
| 6 |
Ketler, Danielle J (13)
|
OHMLY |
6:06.14 |
6:00.87 |
-5.27 |
| 7 |
Zagst, Kailey J (14)
|
DYP |
6:13.18 |
6:01.10 |
-12.08 |
| 8 |
Kovacs, Blake W (13)
|
LERYD |
6:30.16 |
6:10.19 |
-19.97 |
| 9 |
Carson, Abigail M (14)
|
LEGO |
6:16.79 |
6:19.86 |
3.07 |
| 10 |
Henely, Lydia N (14)
|
LERYD |
6:39.38 |
6:24.28 |
-15.10 |
| 11 |
McDaniels, Lainey K (14)
|
LECFYN |
6:26.72 |
6:26.89 |
0.17 |
| 12 |
Noretto, Olivia (13)
|
TUSC |
6:32.64 |
6:28.08 |
-4.56 |
| 13 |
Hobe, Lauren (14)
|
NCY |
6:33.09 |
6:30.46 |
-2.63 |
| 14 |
Sabo, Rebecca (14)
|
LCE |
6:35.89 |
6:30.53 |
-5.36 |
| 15 |
Lanning, Kylie R (13)
|
LEGO |
6:29.58 |
6:30.74 |
1.16 |
| 16 |
Zhao, Jocelyn (13)
|
LEYYN |
6:32.74 |
6:30.80 |
-1.94 |
| 17 |
Hayhurst, Aubrie (13)
|
WAD |
6:42.97 |
6:35.63 |
-7.34 |
| 18 |
Mayer, Madison M (13)
|
LEGYB |
6:32.27 |
6:35.79 |
3.52 |
| 19 |
Avery, Hannah (14)
|
OHLCWE |
6:37.74 |
6:44.86 |
7.12 |
| 20 |
Elliott, Raegan (13)
|
LCE |
6:45.42 |
6:45.80 |
0.38 |