| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lee, Eun Chan (10)
|
ADCPP |
49.32 |
48.13 |
-1.19 |
| 2 |
Lim, Yi Wei (9)
|
ADCPP |
49.11 |
48.93 |
-0.18 |
| 3 |
Yan, Brendan R (9)
|
ADALBS |
51.03 |
49.43 |
-1.60 |
| 4 |
Tong, Yuan (9)
|
ADCPP |
49.45 |
50.22 |
0.77 |
| 5 |
McNair, Finn M (9)
|
ADCLIN |
52.39 |
53.19 |
0.80 |
| 6 |
Larsen, Edwin (9)
|
ADJJSC |
50.73 |
55.57 |
4.84 |
| 7 |
Cao, Austin W (10)
|
ADJJSC |
57.00 |
56.05 |
-0.95 |
| 8 |
Zeliph, Jack H (9)
|
ADALBS |
58.64 |
57.01 |
-1.63 |
| 9 |
Liu, Henry Z (9)
|
ADCPP |
56.94 |
57.85 |
0.91 |
| 10 |
Kropp, Connor T (9)
|
ADEELS |
1:00.83 |
58.04 |
-2.79 |
| 11 |
Mace, Nicholas A (9)
|
ADCPP |
1:02.05 |
58.73 |
-3.32 |
| 12 |
Kennedy, Ian M (9)
|
ADJJSC |
1:08.18 |
1:00.90 |
-7.28 |
| 13 |
Lee, Nathan C (9)
|
ADCPP |
54.36 |
1:01.60 |
7.24 |
| 14 |
Ilardi, Tyler R (9)
|
ADALBS |
1:04.20 |
1:03.05 |
-1.15 |
| 15 |
Mian, Subhaan A (10)
|
ADCLIN |
1:03.76 |
1:05.51 |
1.75 |
| 16 |
Freiberger, Samuel N (10)
|
ADCLIN |
1:01.76 |
1:10.73 |
8.97 |
|
Hughes Robillard, Eric M (10)
|
ADCPP |
1:12.15 |
DQ |
- |
|
Yin, Brayden C (9)
|
ADCPP |
56.60 |
DQ |
- |
|
Zhang, Yangyiyi (10)
|
ADCPP |
1:19.24 |
DQ |
- |
|
Wan, Yuan (9)
|
ADCPP |
1:00.24 |
NS |
- |