| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Keaney, Kasey A (14)
|
ADGUY |
25.75 |
26.88 |
1.13 |
| 2 |
SantaBarbara, Marianna (13)
|
ADDACC |
26.01 |
27.69 |
1.68 |
| 3 |
O'Donnell, Abigael F (14)
|
ADDACC |
27.87 |
27.89 |
0.02 |
| 4 |
Barnhart, Breityn O (14)
|
ADDACC |
29.00 |
28.88 |
-0.12 |
| 5 |
Maier, Madison K (14)
|
ADGLFY |
28.44 |
28.94 |
0.50 |
| 6 |
Buzas, Megan R (13)
|
ADDACC |
29.39 |
29.32 |
-0.07 |
| 7 |
Felton, Emily R (13)
|
ADGLFY |
29.15 |
29.34 |
0.19 |
| 8 |
Rath, Reagan A (14)
|
ADGLFY |
29.84 |
29.83 |
-0.01 |
| 9 |
Coutant, Genevieve K (14)
|
ADGLFY |
32.21 |
31.09 |
-1.12 |
| 10 |
Tanner, Margot R (14)
|
ADGUY |
30.38 |
31.67 |
1.29 |
| 11 |
Lian, Kezhuang (14)
|
ADSSCY |
31.17 |
31.98 |
0.81 |
| 12 |
Rowland, Kylie (14)
|
ADGUY |
30.20 |
32.01 |
1.81 |
| 13 |
Schroder, Abigail J (13)
|
ADSSCY |
32.22 |
32.61 |
0.39 |
| 14 |
Capella, Meghan (13)
|
ADGGFY |
34.14 |
35.18 |
1.04 |
| 15 |
Holt, Caroline (14)
|
ADGGFY |
37.07 |
39.16 |
2.09 |
|
Pacholczak, Anna R (13)
|
ADGUY |
30.20 |
DQ |
- |