| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Giumarra, Sadie E (12)
|
ADGLFY |
28.24 |
27.74 |
-0.50 |
| 2 |
Tennyson, Valerie L (11)
|
ADGLFY |
28.09 |
28.12 |
0.03 |
| 3 |
Jurgelon, Amanda M (12)
|
ADGUY |
29.43 |
28.58 |
-0.85 |
| 4 |
Sellnow, Abigail V (12)
|
ADDACC |
29.20 |
28.74 |
-0.46 |
| 5 |
Steeves, Caroline H (12)
|
ADKING |
28.95 |
28.88 |
-0.07 |
| 6 |
Frisoni, Olympia S (12)
|
ADGLEN |
29.12 |
29.03 |
-0.09 |
| 7 |
Wood, Madeleine T (12)
|
ADSARY |
28.34 |
29.33 |
0.99 |
| 8 |
Hurff, Natasha B (11)
|
ADSARY |
30.45 |
29.58 |
-0.87 |
| 9 |
Bigwarfe, Kayleigh (12)
|
ADBETH |
30.92 |
30.32 |
-0.60 |
| 10 |
Mitchell, Maris C (12)
|
ADDACC |
30.74 |
30.43 |
-0.31 |
| 10 |
Kuo, Lindsay R (11)
|
ADSSCY |
31.24 |
30.43 |
-0.81 |
| 12 |
Thrall, Lydia B (12)
|
ADSARY |
31.44 |
30.44 |
-1.00 |
| 13 |
Cuff, Hayle A (12)
|
ADKING |
30.56 |
30.48 |
-0.08 |
| 14 |
Nowitzki, Katherine R (11)
|
ADKING |
32.45 |
31.34 |
-1.11 |
| 15 |
Gallo, Ella J (12)
|
ADKING |
32.68 |
31.42 |
-1.26 |
| 16 |
Nicastro, Viviana A (12)
|
ADSARY |
31.29 |
31.54 |
0.25 |
| 17 |
De Tuya, Lucia (12)
|
ADBETH |
33.28 |
31.67 |
-1.61 |
| 18 |
Trahan, Ella L (11)
|
ADSARY |
33.97 |
31.68 |
-2.29 |
| 19 |
Kreig, Hannah (12)
|
ADGLEN |
30.98 |
31.90 |
0.92 |
| 20 |
Stanco, Lara-Ann M (12)
|
ADGLFY |
31.80 |
31.92 |
0.12 |
| 21 |
Montanini, Agata (12)
|
ADGUY |
31.96 |
32.05 |
0.09 |
| 22 |
Rosenbaum, Jillian (12)
|
ADGLEN |
31.41 |
32.06 |
0.65 |
| 23 |
Wey, Natalie R (11)
|
ADGUY |
33.50 |
32.29 |
-1.21 |
| 24 |
Chorzempa, Abigail C (11)
|
ADKING |
32.55 |
32.39 |
-0.16 |
| 24 |
Bonesteel, Jana M (11)
|
ADGLFY |
33.55 |
32.39 |
-1.16 |
| 26 |
Connolly, Caroline A (11)
|
ADKING |
32.53 |
32.40 |
-0.13 |
| 27 |
Bowman, Hannah (11)
|
ADGUY |
33.59 |
32.47 |
-1.12 |
| 28 |
Van Woert, Maura M (12)
|
ADGUY |
33.39 |
32.69 |
-0.70 |
| 29 |
Ormsby, Mikayla (12)
|
ADGLEN |
34.00 |
32.92 |
-1.08 |
| 30 |
Crowd, Arianna N (12)
|
ADGLFY |
32.32 |
32.97 |
0.65 |
| 31 |
Bearor, McKenna M (11)
|
ADGLFY |
33.03 |
33.01 |
-0.02 |
| 32 |
Goffioul, Clara Marie A (11)
|
ADSARY |
33.35 |
33.07 |
-0.28 |
| 33 |
Borski, Hannah P (12)
|
ADDACC |
33.97 |
33.26 |
-0.71 |
| 34 |
Huang, Madison Z (11)
|
ADSSCY |
32.80 |
33.53 |
0.73 |
| 35 |
Zebrowski, Olivia G (12)
|
ADSARY |
33.18 |
33.75 |
0.57 |
| 36 |
Kelly, Anna K (12)
|
ADGUY |
33.83 |
34.20 |
0.37 |
| 37 |
Salisbury, Emily (12)
|
ADGUY |
33.48 |
35.03 |
1.55 |
| 38 |
Druskin, Casey (12)
|
ADKING |
33.21 |
35.65 |
2.44 |
| 39 |
Akley, Ella G (11)
|
ADGBY |
33.49 |
35.90 |
2.41 |