| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Adams, Michelle E (7)
|
ADCPP |
51.85 |
50.29 |
-1.56 |
| 2 |
Cho, Anna Y (8)
|
ADALBS |
52.18 |
51.57 |
-0.61 |
| 3 |
Cao, Aimee W (8)
|
ADJJSC |
55.97 |
52.55 |
-3.42 |
| 4 |
Stewart Fraser, Thornton R (8)
|
ADALBS |
52.54 |
52.93 |
0.39 |
| 5 |
Hopke, Lillian G (6)
|
ADCPP |
59.43 |
58.80 |
-0.63 |
| 6 |
O'Donnell, Bridgett M (8)
|
ADEELS |
1:09.51 |
1:00.39 |
-9.12 |
| 7 |
Pacinella, Juliann F (8)
|
ADCPP |
1:05.08 |
1:01.65 |
-3.43 |
| 8 |
Miller, Isabella M (8)
|
ADCPP |
1:08.70 |
1:03.19 |
-5.51 |
| 9 |
Yager, Myah Y (8)
|
ADCANA |
1:07.24 |
1:04.58 |
-2.66 |
| 10 |
Zhou, Yixuan (8)
|
ADCPP |
1:05.86 |
1:06.07 |
0.21 |
| 11 |
Currier, Lillian G (7)
|
ADCPP |
1:10.52 |
1:08.73 |
-1.79 |
| 12 |
Harpootlian, Leah C (7)
|
ADCPP |
1:10.03 |
1:09.56 |
-0.47 |
|
Huang, Grace X (8)
|
ADCPP |
1:20.55 |
DQ |
- |
|
McAllister, Riley A (7)
|
ADJJSC |
1:21.04 |
DQ |
- |