| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Felton, Eleanor V (17)
|
ADDACC |
53.07 |
54.97 |
1.90 |
| 2 |
Koeferl, Alexandra M (16)
|
ADGLEN |
58.33 |
57.14 |
-1.19 |
| 3 |
Gilbert, Portia M (15)
|
ADGLEN |
57.42 |
57.44 |
0.02 |
| 4 |
Peck, Alexa E (15)
|
ADKING |
59.65 |
58.07 |
-1.58 |
| 5 |
Cartwright, Harper V (15)
|
ADGBY |
1:00.96 |
1:01.08 |
0.12 |
| 6 |
Barnhart, Breityn O (15)
|
ADDACC |
1:02.34 |
1:02.20 |
-0.14 |
| 7 |
Rizzo, Emma R (17)
|
ADGLOY |
1:01.80 |
1:03.27 |
1.47 |
| 8 |
Frenyea, Sophia J (16)
|
ADGLOY |
1:00.86 |
1:03.50 |
2.64 |
| 9 |
Coutant, Genevieve K (15)
|
ADGLFY |
1:02.07 |
1:04.49 |
2.42 |
| 10 |
Cromer, Olivia A (17)
|
ADGLOY |
1:04.28 |
1:05.06 |
0.78 |
| 11 |
Rutkowski, Janna K (15)
|
ADKING |
1:00.13 |
1:05.25 |
5.12 |
| 12 |
Smith, Sydney (15)
|
ADBETH |
1:06.17 |
1:07.12 |
0.95 |
| 12 |
Norris, Julia M (15)
|
ADGLOY |
1:08.35 |
1:07.12 |
-1.23 |
| 14 |
Adeson, Elliana D (15)
|
ADGLFY |
1:00.85 |
1:07.74 |
6.89 |
| 15 |
Rizzo, Abigail E (15)
|
ADGLOY |
1:07.84 |
1:09.14 |
1.30 |
|
DeFrancisco, Celia E (15)
|
ADGUY |
1:06.90 |
DQ |
- |