| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mack, Katelyn G (15)
|
PVNCAP |
49.75 |
50.64 |
0.89 |
| 2 |
Heilbrun, Madeline C (16)
|
PVNCAP |
52.41 |
52.30 |
-0.11 |
| 3 |
Gati, Isabella G (16)
|
PVNCAP |
51.97 |
52.70 |
0.73 |
| 4 |
Kuhlkin, Elizabeth C (14)
|
PVNCAP |
53.41 |
53.64 |
0.23 |
| 5 |
Davis, Sofia C (15)
|
PVNCAP |
53.12 |
53.97 |
0.85 |
| 6 |
McCulla, Elizabeth C (17)
|
PVNCAP |
53.97 |
54.53 |
0.56 |
| 7 |
MacMillan, Allie C (15)
|
PVNCAP |
54.06 |
54.68 |
0.62 |
| 8 |
Fu, Joanne H (17)
|
PVNCAP |
53.29 |
55.23 |
1.94 |
| 9 |
Bartholomew, Olivia (17)
|
PVUMAC |
1:03.42 |
1:06.63 |
3.21 |
| 10 |
Hughes, Danielle M (9)
|
PVAAC |
1:12.64 |
1:11.55 |
-1.09 |
| 11 |
Baterdene, Yalguun (10)
|
PVAAC |
1:23.51 |
1:23.95 |
0.44 |
| 12 |
Nagle, Zoe K (11)
|
PVAAC |
1:36.62 |
1:26.54 |
-10.08 |
|
Plummer, Tate S (11)
|
PVAAC |
1:22.63 |
NS |
- |
|
Ma, Leaya L (14)
|
PVNCAP |
54.08 |
NS |
- |