| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nebraska, Leah K (12)
|
OHDORC |
2:29.12 |
2:22.51 |
-6.61 |
| 2 |
Bzdafka, Amira G (12)
|
OHOSSC |
2:30.54 |
2:23.33 |
-7.21 |
| 3 |
Sturgeon, Lydia N (12)
|
OHPHST |
2:35.99 |
2:23.40 |
-12.59 |
| 4 |
Hall, Rio I (12)
|
OHOSSC |
2:28.92 |
2:25.04 |
-3.88 |
| 5 |
Visurakapalli, Shanti A (12)
|
OHFRCE |
2:28.92 |
2:25.36 |
-3.56 |
| 6 |
Boccio, Mia A (12)
|
OHOSSC |
2:32.64 |
2:28.03 |
-4.61 |
| 7 |
Root, Rhiannon K (12)
|
OHOSSC |
2:29.95 |
2:28.12 |
-1.83 |
| 8 |
Yarbrough, Chelsea C (11)
|
OHUSA |
3:16.28 |
2:31.39 |
-44.89 |
| 9 |
Bowman, Emma O (11)
|
OHUSA |
2:35.19 |
2:35.48 |
0.29 |
| 10 |
Goodrich, Abigail L (11)
|
OHOSSC |
2:36.00 |
2:37.83 |
1.83 |
| 11 |
Kincaid, Madison E (12)
|
OHUSA |
3:00.19 |
2:51.58 |
-8.61 |
| 12 |
Alsko, Leah E (12)
|
OHUSA |
3:00.19 |
2:55.51 |
-4.68 |
| 13 |
Cornelius, Savannah M (12)
|
OHBKYS |
2:59.62 |
3:02.91 |
3.29 |