| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rising, London A (10)
|
MILCY |
58.85 |
58.77 |
-0.08 |
| 2 |
McCuiston, Charlie H (10)
|
MIBBD |
1:01.98 |
1:01.02 |
-0.96 |
| 3 |
Whan, Ian A (10)
|
MIBBD |
59.71 |
1:02.43 |
2.72 |
| 4 |
Wachauf, Caleb J (10)
|
OHYWSC |
1:02.05 |
1:03.08 |
1.03 |
| 5 |
Mongomery, Colten (9)
|
OHSHLB |
1:01.81 |
1:03.36 |
1.55 |
| 6 |
Rose, Will (10)
|
KYNEY |
1:06.00 |
1:04.03 |
-1.97 |
| 7 |
Mignery, Luke E (10)
|
OHPCY |
1:02.74 |
1:04.06 |
1.32 |
| 8 |
Menne, Carter J (10)
|
OHABLY |
1:05.62 |
1:04.93 |
-0.69 |
| 9 |
Stebbins, Nathan M (10)
|
MIBBD |
1:03.97 |
1:05.61 |
1.64 |
| 10 |
Kyre, Nathan G (10)
|
OHKEY |
1:04.64 |
1:05.72 |
1.08 |
| 11 |
Barasch, Nathan T (10)
|
OHFMST |
1:04.08 |
1:05.76 |
1.68 |
| 12 |
Rogers, Toby (10)
|
OHLCCY |
1:06.69 |
1:06.50 |
-0.19 |
| 13 |
May, Carson S (10)
|
LECFYN |
1:06.24 |
1:06.73 |
0.49 |
| 14 |
Boh, Andrew M (10)
|
OHRCDB |
1:03.98 |
1:06.92 |
2.94 |
| 15 |
Rich, Colton J (9)
|
MIFLY |
1:06.58 |
1:07.11 |
0.53 |
| 16 |
Watters, Cooper (10)
|
LEAYAA |
1:07.35 |
1:08.18 |
0.83 |
| 17 |
Sinno, Nabil (10)
|
OHABLY |
1:06.91 |
1:08.23 |
1.32 |
| 18 |
Schultz, Carter (9)
|
INYMMI |
1:07.22 |
1:08.27 |
1.05 |
| 19 |
Ott, Carter W (10)
|
OHLAKY |
1:07.71 |
1:08.63 |
0.92 |
| 20 |
Luebke, Gavin (9)
|
OHAMFY |
1:07.13 |
1:08.72 |
1.59 |
| 21 |
Stenger, Jacob A (10)
|
OHABLY |
1:07.57 |
1:09.05 |
1.48 |
| 22 |
Bucko, Parker S (9)
|
OHFMST |
1:08.08 |
1:09.08 |
1.00 |
| 23 |
Mccall, Henry E (10)
|
OHKEY |
1:06.74 |
1:09.84 |
3.10 |
| 24 |
Snyder, Joseph D (10)
|
OHPCY |
1:07.66 |
1:14.68 |
7.02 |
|
Smith, Talan J (9)
|
OHDYP |
1:07.28 |
DQ |
- |
|
Fletcher, Jr, Aaron O (9)
|
MINOW |
1:06.62 |
NS |
- |
|
Jansen, Aidan N (10)
|
OHCY |
1:03.68 |
NS |
- |