| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wisler, Alex J (17)
|
OHLTPY |
23.53 |
22.68 |
-0.85 |
| 2 |
Bottomley, Robert T (16)
|
LERYD |
24.64 |
23.49 |
-1.15 |
| 3 |
LeTourneau, Avery J (16)
|
MIRAYS |
24.26 |
23.57 |
-0.69 |
| 4 |
Bently, John E (17)
|
OHLTPY |
23.93 |
23.74 |
-0.19 |
| 5 |
Dunlany, Matthew (17)
|
LEYYN |
23.72 |
23.77 |
0.05 |
| 6 |
Poletta, Daniel L (18)
|
LEGYB |
25.06 |
24.03 |
-1.03 |
| 7 |
Villalon, Kendrick P (17)
|
MISYS |
24.52 |
24.20 |
-0.32 |
| 8 |
Gehring, Andrew G (16)
|
OHLTPY |
24.62 |
24.23 |
-0.39 |
| 9 |
Orozco, Mitch D (18)
|
OHMCY |
25.12 |
24.30 |
-0.82 |
| 10 |
Glennon, Kevin C (14)
|
OHCY |
24.91 |
24.34 |
-0.57 |
| 11 |
Carter, John M (17)
|
WVPKB |
24.53 |
24.35 |
-0.18 |
| 12 |
Tate, Phillip J (18)
|
OHHURY |
24.21 |
24.43 |
0.22 |
| 13 |
Sturgill, Aidan (17)
|
KYBYB |
24.63 |
24.47 |
-0.16 |
| 14 |
Menchhofer, Bryce R (13)
|
OHBASH |
25.07 |
24.61 |
-0.46 |
| 15 |
Iversen, Isaac C (16)
|
OHLTPY |
25.40 |
24.67 |
-0.73 |
| 16 |
Bauer, Nicholas C (17)
|
OHTCY |
25.12 |
24.69 |
-0.43 |
| 17 |
Mcguire, Gregory D (15)
|
OHHURY |
25.27 |
24.70 |
-0.57 |
| 18 |
Schneider, Patrick L (18)
|
KYBYB |
24.95 |
24.72 |
-0.23 |
| 18 |
Doerrer, Mason j (17)
|
LEGYB |
25.14 |
24.72 |
-0.42 |
| 20 |
Aldewereld, Luca J (16)
|
LERYD |
28.26 |
24.77 |
-3.49 |
| 21 |
Camy, Julien J (18)
|
MIRAYS |
24.24 |
24.81 |
0.57 |
| 22 |
Grubbs, Elijah M (16)
|
OHLTPY |
24.91 |
24.86 |
-0.05 |
| 23 |
Hart, Jacob E (17)
|
MINOW |
24.67 |
25.04 |
0.37 |
| 24 |
Dixon, Geoffrey A (17)
|
OHLYST |
25.03 |
25.06 |
0.03 |
| 25 |
Spees, Xander (15)
|
OHAMFY |
25.55 |
25.09 |
-0.46 |
| 26 |
Spyker, Charlie J (16)
|
OHLYSC |
24.91 |
25.14 |
0.23 |
| 27 |
Garboden, Jack C (16)
|
OHBASH |
24.94 |
25.20 |
0.26 |
| 28 |
Betscakos, Maximus (18)
|
LEGYB |
24.98 |
25.24 |
0.26 |
| 29 |
Heberle, Luke D (16)
|
LERYD |
25.14 |
25.28 |
0.14 |
| 30 |
McAlister, Max A (15)
|
OHLTPY |
25.43 |
25.31 |
-0.12 |
| 31 |
DeBoer, Andrew H (17)
|
MIRAYS |
25.57 |
25.42 |
-0.15 |
| 32 |
Ingram, Dylan J (16)
|
OHLTPY |
25.49 |
25.46 |
-0.03 |
| 33 |
Lucas, Evan (15)
|
OHBASH |
25.29 |
25.50 |
0.21 |
| 34 |
Stackpole, Jordan C (17)
|
LEYYN |
25.44 |
25.56 |
0.12 |
| 35 |
Nelson, Aidan D (16)
|
MIBBD |
25.43 |
25.67 |
0.24 |
| 36 |
Blum, Andrew (16)
|
OHRCY |
25.57 |
25.72 |
0.15 |
| 37 |
Pan, Andrew A (15)
|
MIFFYS |
24.38 |
25.74 |
1.36 |
| 38 |
Davidson, Wes (16)
|
OHPUT |
25.38 |
25.93 |
0.55 |
| 39 |
Villarreal, Ricky (18)
|
OHRCY |
25.35 |
25.94 |
0.59 |
|
Fabiano, Alexander A (17)
|
MIRAYS |
25.01 |
NS |
- |