| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chambers, Isabella L (12)
|
FGPST |
32.45 |
32.43 |
-0.02 |
| 2 |
Sierra, Micaela (12)
|
FGUN |
36.36 |
32.58 |
-3.78 |
| 3 |
Fernandez, Michelle A (12)
|
FGHSC |
33.78 |
32.73 |
-1.05 |
| 4 |
Botting, Riley E (12)
|
FGCSSC |
33.29 |
32.80 |
-0.49 |
| 5 |
Kaplan, Kate S (12)
|
FGCCPR |
34.46 |
33.40 |
-1.06 |
| 6 |
Torres, Victoria C (12)
|
FGSOFL |
34.91 |
33.81 |
-1.10 |
| 7 |
Hernandez, Brianna (12)
|
FGYSF |
33.72 |
34.00 |
0.28 |
| 8 |
Araujo, Rafaella S (11)
|
FGUN |
35.00 |
34.08 |
-0.92 |
| 9 |
Miller, Erin G (11)
|
FGSAS |
35.48 |
34.37 |
-1.11 |
| 10 |
Ruggiere, Kai K (12)
|
FGECAC |
34.74 |
34.68 |
-0.06 |
| 11 |
Golding, Sally C (12)
|
FGCCPR |
35.13 |
35.07 |
-0.06 |
| 12 |
Torretta, Aria A (12)
|
FGGRSC |
36.53 |
35.09 |
-1.44 |
| 13 |
Yates, Melody (12)
|
FGPCS |
34.88 |
35.21 |
0.33 |
| 14 |
McLemore, Tawannah P (11)
|
FGBEST |
34.14 |
35.35 |
1.21 |
| 15 |
Escudero, Mia F (12)
|
FGSOFL |
36.48 |
35.45 |
-1.03 |
| 16 |
Osorio, Sabrina (12)
|
FGSOFL |
37.28 |
36.17 |
-1.11 |
| 17 |
Roldan, Adriana (12)
|
FGGRSC |
35.52 |
36.28 |
0.76 |
| 18 |
Soldani, Lily E (11)
|
FGPCS |
37.47 |
36.32 |
-1.15 |
| 19 |
Levin, Rebeca (12)
|
FGSF |
37.83 |
36.55 |
-1.28 |
| 20 |
Vank, Valerie (11)
|
FGSOFL |
35.21 |
36.70 |
1.49 |
| 21 |
Hanssen, Alexa J (12)
|
FGJDST |
37.70 |
36.85 |
-0.85 |
| 22 |
Proscia, Maris A (11)
|
FGFAST |
37.02 |
36.86 |
-0.16 |
| 23 |
Marino, Yazmin A (12)
|
FGYSF |
37.30 |
37.36 |
0.06 |
| 24 |
Pierre-Antoine, Eva V (12)
|
FGCCPR |
37.44 |
37.47 |
0.03 |
| 25 |
Rosano Sam, Anaveronica (11)
|
FGCM |
37.89 |
37.51 |
-0.38 |
| 26 |
Gonzalez, Melissa (12)
|
FGAZFL |
37.59 |
37.95 |
0.36 |
| 27 |
Frazier, Sara M (12)
|
FGFFSC |
37.66 |
38.28 |
0.62 |
| 28 |
Perez, Casey (11)
|
FGBMHG |
37.48 |
38.49 |
1.01 |
| 29 |
Smith, Corley F (12)
|
FGFKSC |
37.66 |
39.52 |
1.86 |
|
Egizi, Bella M (11)
|
FGPCS |
37.18 |
NS |
- |