| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hackett, Creed T (10)
|
NICSST |
43.14 |
38.36 |
-4.78 |
| 2 |
Cox, Oliver S (10)
|
NIHAWK |
41.93 |
40.92 |
-1.01 |
| 3 |
Arron, Cooper J (9)
|
NIUNAC |
44.83 |
42.20 |
-2.63 |
| 4 |
Lucero, Cody (10)
|
NICSST |
49.43 |
42.58 |
-6.85 |
| 5 |
Tran, Thai D (9)
|
NIHAWK |
44.23 |
45.28 |
1.05 |
| 6 |
Brenen-Buseck, Samuel R (10)
|
NICSST |
46.66 |
46.70 |
0.04 |
| 7 |
Mayoros, Jan R (10)
|
NICSST |
52.10 |
48.68 |
-3.42 |
| 8 |
Segal, Samuel M (10)
|
NIUN |
56.31 |
49.12 |
-7.19 |
| 9 |
Khrapko, Denis (10)
|
NIHAWK |
|
49.31 |
- |
| 10 |
Leonard, Joshua D (9)
|
NIWAAC |
1:00.22 |
49.37 |
-10.85 |
| 11 |
McGovern, Colin M (9)
|
NIUNAC |
|
49.72 |
- |
| 12 |
Potter, Jackson J (10)
|
NICSST |
1:02.41 |
50.43 |
-11.98 |
| 13 |
Gorman, Ryan P (9)
|
NIWAAC |
53.14 |
50.52 |
-2.62 |
| 14 |
Lewis, Parker M (9)
|
NIUNAC |
51.87 |
50.56 |
-1.31 |
| 15 |
Sullivan, Devin R (9)
|
NIHHTA |
1:00.60 |
52.03 |
-8.57 |
| 16 |
Mangoba, Zachary A (9)
|
NIISWM |
50.92 |
52.11 |
1.19 |
| 17 |
Wood, Andrew G (10)
|
NIHHTA |
55.54 |
53.71 |
-1.83 |
| 18 |
Hong, Mark (9)
|
NIHAWK |
1:07.74 |
53.77 |
-13.97 |
| 19 |
Lawson, Nathan M (10)
|
NIHHTA |
|
54.44 |
- |
| 20 |
Law, Linus C (10)
|
NIHHTA |
|
54.75 |
- |
| 21 |
Brackel, Alex F (9)
|
NIWAAC |
53.53 |
54.97 |
1.44 |
| 22 |
Hartke, Grayson L (9)
|
NIHHTA |
|
55.59 |
- |
| 23 |
Williams, Mason F (9)
|
NIUNAC |
|
55.68 |
- |
| 24 |
Fryburger, Grant W (10)
|
NIGGST |
|
56.62 |
- |
| 25 |
Tarbox, Nathan J (10)
|
NIUNAC |
1:00.51 |
56.97 |
-3.54 |
| 26 |
Wood, Keenan J (9)
|
NICSST |
|
58.45 |
- |
| 27 |
Anderson, Koen T (8)
|
NIISWM |
53.27 |
58.73 |
5.46 |
| 28 |
Clark, Aidan J (8)
|
NIWAAC |
|
59.03 |
- |
| 29 |
Carter, Kaleb J (10)
|
NICVSC |
|
59.08 |
- |
| 30 |
Sincock, Brayden V (10)
|
NICSST |
|
59.31 |
- |
| 31 |
Reimsnyder, Marvin W (9)
|
NIHAWK |
|
59.61 |
- |
| 32 |
Littrell, Alexander W (10)
|
NIHHTA |
|
1:01.16 |
- |
| 33 |
Carling, Raleigh J (10)
|
NIHHTA |
1:06.48 |
1:01.35 |
-5.13 |
| 34 |
Murphy, Charlie J (10)
|
NICSST |
|
1:02.76 |
- |
| 35 |
Nale, Joshua D (9)
|
NIUNAC |
|
1:04.83 |
- |
| 36 |
Russell, Christopher M (10)
|
NIHAWK |
1:18.74 |
1:05.76 |
-12.98 |
| 37 |
Driscoll, Lucas (10)
|
NIHHTA |
|
1:06.06 |
- |
| 38 |
Carbone, Nicholas L (9)
|
NIGGST |
|
1:08.36 |
- |
| 39 |
Wu, Winston X (9)
|
NIHAWK |
|
1:10.99 |
- |
| 40 |
Jiang, Rui (9)
|
NIHAWK |
|
1:18.58 |
- |
|
Tran, Thang D (7)
|
NIHAWK |
|
DQ |
- |
|
Cho, Seojun (10)
|
NIHAWK |
44.78 |
DQ |
- |
|
Armstrong, Liam M (10)
|
NIUNAC |
|
NS |
- |
|
Ankam, Ronel P (9)
|
NIWAAC |
|
NS |
- |