| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Manganiello, Makenna M (10)
|
ILSAGE |
44.83 |
43.76 |
-1.07 |
| 2 |
Dejworek, Daisy (10)
|
ILSAGE |
43.37 |
43.78 |
0.41 |
| 3 |
Zimmerman, Natalie L (10)
|
ILSAGE |
43.70 |
43.79 |
0.09 |
| 4 |
Kahn, Brooklyn F (9)
|
ILSAGE |
47.33 |
46.88 |
-0.45 |
| 5 |
Lowery, Emily M (9)
|
ILVY |
49.91 |
47.71 |
-2.20 |
| 6 |
Wetzel, Eva E (9)
|
ILSAGE |
47.85 |
48.68 |
0.83 |
| 7 |
Wang, Lily Z (9)
|
ILSAGE |
48.40 |
49.07 |
0.67 |
| 8 |
Smith, Taylor M (10)
|
ILFVFY |
51.80 |
49.30 |
-2.50 |
| 9 |
Manganiello, Maeve E (9)
|
ILSAGE |
50.18 |
49.61 |
-0.57 |
| 10 |
Jordan, Victoria R (10)
|
ILFVFY |
51.79 |
50.97 |
-0.82 |
| 11 |
Urbates, Nora E (9)
|
ILSAGE |
53.10 |
51.07 |
-2.03 |
| 12 |
Duncan, Madison A (9)
|
ILSAGE |
50.04 |
52.19 |
2.15 |
| 13 |
Villa, Melany (9)
|
ILFVFY |
1:06.13 |
55.01 |
-11.12 |
| 14 |
Manganiello, Madelyn M (10)
|
ILSAGE |
57.43 |
57.33 |
-0.10 |
| 15 |
Bown, Alexandria B (9)
|
ILBVDY |
|
1:09.57 |
- |
| 16 |
Gibson, Sophia G (9)
|
ILSAGE |
1:10.63 |
1:13.01 |
2.38 |
|
Howerton, Isabelle C (9)
|
ILFVFY |
|
DQ |
- |