| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ware, Erin C (14)
|
GUTTST |
5:51.51 |
5:38.32 |
-13.19 |
| 2 |
Callahan, Isabelle A (13)
|
GUCFSC |
5:57.62 |
5:53.87 |
-3.75 |
| 3 |
Lundquist, Anna K (14)
|
GUSWIM |
6:09.50 |
5:55.39 |
-14.11 |
| 4 |
Jordan, Lilian R (13)
|
GUBATS |
6:00.12 |
5:58.49 |
-1.63 |
| 5 |
Mao, Ella C (13)
|
GUSWIM |
6:02.19 |
6:03.06 |
0.87 |
| 6 |
Salge, Emmilee E (13)
|
GUNOCH |
6:33.69 |
6:03.63 |
-30.06 |
| 7 |
Curran, Trinity E (13)
|
GUMAC |
|
6:03.64 |
- |
| 8 |
Powers, Brooke C (13)
|
GUDADS |
|
6:04.87 |
- |
| 9 |
Briggs, Abigail (13)
|
GUTYES |
6:07.31 |
6:08.06 |
0.75 |
| 10 |
Grzyb, Kalle E (13)
|
GUPACK |
6:08.24 |
6:33.22 |
24.98 |
| 11 |
Cole, Kaylee J (14)
|
GUVSST |
5:48.93 |
6:33.32 |
44.39 |
| 12 |
Naramore, Samantha L (13)
|
GUAMBU |
|
6:42.61 |
- |