| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lopez, Sarah (14)
|
GUSSAN |
2:26.25 |
2:32.30 |
6.05 |
| 2 |
Miller, Hayden K (14)
|
GUCFSC |
2:34.15 |
2:32.96 |
-1.19 |
| 3 |
Busch, Olivia M (14)
|
GUMAC |
2:29.55 |
2:35.75 |
6.20 |
| 4 |
Culberson, Sarah G (14)
|
GUMAC |
2:27.09 |
2:38.78 |
11.69 |
| 5 |
Riederer, Evlin M (13)
|
GUSSAN |
2:44.14 |
2:39.58 |
-4.56 |
| 6 |
Osorio, Maria V (13)
|
GUCFSC |
2:52.32 |
2:45.92 |
-6.40 |
| 7 |
Guglielmello, Emma S (14)
|
GUMAC |
2:42.68 |
2:47.57 |
4.89 |
| 8 |
Smoker, Ella J (13)
|
GUCFSC |
2:53.70 |
2:52.53 |
-1.17 |
| 9 |
Greeney, Paige M (14)
|
GUSSAN |
2:59.56 |
2:53.43 |
-6.13 |
| 10 |
Devens, Ellie V (13)
|
GUCFSC |
2:53.16 |
2:54.10 |
0.94 |
| 11 |
Schlimper, Christa A (14)
|
GUCFSC |
3:06.79 |
2:55.45 |
-11.34 |
| 12 |
Dao, Venus C (14)
|
GUCFSC |
2:56.79 |
3:00.86 |
4.07 |
| 13 |
Culberson, Claire J (14)
|
GUMAC |
|
3:09.43 |
- |
| 14 |
Salge, Emmilee E (13)
|
GUNOCH |
2:47.93 |
3:16.44 |
28.51 |
| 15 |
Barrera, Lillyanna (14)
|
GUNOCH |
3:53.64 |
3:28.84 |
-24.80 |