| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gaier, Aiden J (16)
|
OHLTPY |
22.51 |
22.52 |
0.01 |
| 2 |
Lloyd, Jahvon A (17)
|
OHHURY |
23.71 |
22.63 |
-1.08 |
| 3 |
Chi, Joshua E (17)
|
OHHURY |
23.59 |
23.09 |
-0.50 |
| 4 |
Holst, Philip A (18)
|
OHLTPY |
23.25 |
23.80 |
0.55 |
| 5 |
Edwardson, Clayton M (16)
|
OHLTPY |
24.36 |
23.88 |
-0.48 |
| 6 |
De Mesa, Raul S (17)
|
OHGCY |
24.91 |
23.92 |
-0.99 |
| 7 |
Patterson, Christopher C (16)
|
OHLTPY |
24.84 |
24.04 |
-0.80 |
| 8 |
Antonelli, Jacob (16)
|
OHGCY |
24.48 |
24.39 |
-0.09 |
| 9 |
Tseng, Chih-Hsiang J (18)
|
OHLTPY |
24.05 |
24.49 |
0.44 |
| 10 |
Oralov, Alex S (17)
|
OHHURY |
25.54 |
24.72 |
-0.82 |
| 11 |
Kilrain, Connor P (16)
|
OHLTPY |
24.50 |
24.90 |
0.40 |
| 12 |
Howard, Lance G (15)
|
OHGCY |
25.49 |
25.48 |
-0.01 |
| 13 |
Stout, Luke (15)
|
OHPICY |
27.03 |
26.64 |
-0.39 |
| 14 |
Sebenoler, Thomas D (15)
|
OHLTPY |
29.35 |
27.67 |
-1.68 |
| 15 |
Loughrey, Carson M (15)
|
OHLTPY |
27.97 |
28.36 |
0.39 |
| 16 |
Bueter, Brayden A (15)
|
OHGCY |
31.83 |
30.88 |
-0.95 |