| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
He, Kristin (15)
|
IADSMY |
34.93 |
33.16 |
-1.77 |
| 2 |
Peck, Anja C (13)
|
IADSMY |
33.80 |
33.99 |
0.19 |
| 3 |
Lackmann, Torin A (16)
|
IADSMY |
|
34.73 |
- |
| 4 |
Hanna, Makayla E (15)
|
IADSMY |
35.17 |
35.73 |
0.56 |
| 5 |
Boehlert, Lydia J (15)
|
IADSMY |
35.00 |
35.88 |
0.88 |
| 6 |
Van Quathem, Avery E (13)
|
IADSMY |
36.04 |
36.05 |
0.01 |
| 7 |
Thompson, Katherine G (14)
|
IADSMY |
37.70 |
38.33 |
0.63 |
| 8 |
Coates, Payten L (14)
|
IADSMY |
41.48 |
39.14 |
-2.34 |
| 9 |
McCrary, Sara C (13)
|
IADSMY |
38.09 |
40.28 |
2.19 |
| 10 |
Green, Ashley S (13)
|
IADSMY |
42.35 |
41.89 |
-0.46 |
| 11 |
Miller, Josie (14)
|
IAWASH |
42.61 |
42.85 |
0.24 |
|
Sieren, Greta (13)
|
IAWASH |
40.80 |
NS |
- |
|
Todd, Ellie (17)
|
IAWASH |
|
NS |
- |