| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cerda, Lily G (11)
|
IADSMY |
31.31 |
31.09 |
-0.22 |
| 2 |
Boyer, Ellianna (12)
|
IABUR |
34.38 |
32.19 |
-2.19 |
| 3 |
Silvay, Olivia G (11)
|
IADSMY |
34.52 |
33.95 |
-0.57 |
| 4 |
White, Victoria (11)
|
IANEWT |
33.58 |
34.05 |
0.47 |
| 5 |
Kuhlmann, Hayden (11)
|
IAALG |
34.37 |
34.39 |
0.02 |
| 6 |
Wakely, Karena Z (12)
|
IADSMY |
34.88 |
34.64 |
-0.24 |
| 7 |
Dinkin, Cassidy M (12)
|
IADSMY |
37.09 |
35.79 |
-1.30 |
| 8 |
Xu, Hannah V (11)
|
IADSMY |
36.50 |
36.67 |
0.17 |
| 9 |
Jeffryes, Lily F (11)
|
IADSMY |
40.31 |
39.14 |
-1.17 |
| 10 |
Smith, Isabella A (11)
|
IADSMY |
37.99 |
39.37 |
1.38 |
| 11 |
Prueter, Haylee M (11)
|
IADSMY |
41.00 |
39.78 |
-1.22 |
| 12 |
McDowell, Alayna (11)
|
IABUR |
40.47 |
39.88 |
-0.59 |
| 13 |
Zheng, Emma J (12)
|
IADSMY |
|
43.24 |
- |
| 14 |
Sutherland, Lauren R (11)
|
IADSMY |
47.56 |
47.41 |
-0.15 |
| 15 |
Bumgardner, Sophie R (12)
|
IADSMY |
44.38 |
47.49 |
3.11 |