| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Clewell, Nicholas R (11)
|
IADSMY |
40.63 |
38.86 |
-1.77 |
| 2 |
Schechinger, Abe (12)
|
IANVFY |
41.51 |
39.71 |
-1.80 |
| 3 |
Brownsberger, Tate T (12)
|
IADSMY |
45.68 |
42.01 |
-3.67 |
| 4 |
Scuffham, Parker S (11)
|
IADSMY |
43.05 |
42.98 |
-0.07 |
| 5 |
Tuyls, Kellen (12)
|
IADSMY |
43.13 |
43.34 |
0.21 |
| 6 |
Quist, Hunter (11)
|
IANVFY |
46.52 |
44.76 |
-1.76 |
| 7 |
Kearney, Noah J (11)
|
IADSMY |
48.14 |
45.74 |
-2.40 |
| 8 |
Wilson, Parker J (11)
|
IADSMY |
44.95 |
45.95 |
1.00 |
| 9 |
May, Michael J (11)
|
IADSMY |
50.45 |
49.04 |
-1.41 |
| 10 |
Hammond, Alexander E (11)
|
IADSMY |
55.11 |
50.50 |
-4.61 |
| 11 |
Weston, Ethan (11)
|
IABBST |
50.83 |
50.78 |
-0.05 |
| 12 |
Miller, Owen (11)
|
IADSMY |
55.93 |
51.40 |
-4.53 |
|
Wilson, Andrew H (12)
|
IADSMY |
53.43 |
DQ |
- |