| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cerda, Lily G (11)
|
IADSMY |
38.26 |
37.76 |
-0.50 |
| 2 |
Spanovic, Nina T (11)
|
IADSMY |
37.94 |
37.91 |
-0.03 |
| 3 |
Olson, Kacy L (12)
|
IADSMY |
40.68 |
39.84 |
-0.84 |
| 4 |
Wakely, Karena Z (12)
|
IADSMY |
42.40 |
40.75 |
-1.65 |
| 5 |
Crosser, Kate (11)
|
IACRSP |
43.00 |
42.42 |
-0.58 |
| 6 |
Ruan, Catherine C (11)
|
IADSMY |
45.07 |
46.82 |
1.75 |
| 7 |
Nanaziashvili, Sasha (11)
|
IADSMY |
50.55 |
48.84 |
-1.71 |
| 8 |
Porter, Claira L (11)
|
IADSMY |
|
52.66 |
- |
| 9 |
Baker, Isabelle M (11)
|
IADSMY |
49.39 |
54.50 |
5.11 |
| 10 |
Schulte, Mia J (11)
|
IADSMY |
1:01.97 |
57.96 |
-4.01 |
| 11 |
Guiter, Reagan (11)
|
SPS |
|
1:18.23 |
- |
|
van den Berge, Marley J (11)
|
IADSMY |
|
NS |
- |