| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ward, Kylah (10)
|
MALYM |
41.57 |
43.16 |
1.59 |
| 2 |
Dougherty, Renee T (10)
|
MARKRY |
44.84 |
45.42 |
0.58 |
| 3 |
Persch, Hailey G (10)
|
MABCY |
47.35 |
46.07 |
-1.28 |
| 4 |
Luddecke, Megan (9)
|
HATY |
45.79 |
46.09 |
0.30 |
| 5 |
Seng, Rachehny N (10)
|
MALYM |
46.35 |
46.71 |
0.36 |
| 6 |
Richardson, Peyton E (10)
|
MARKRY |
47.83 |
47.15 |
-0.68 |
| 7 |
Ohm, Kamryn J (10)
|
MARKRY |
46.67 |
47.68 |
1.01 |
| 8 |
Stewart, Ava (10)
|
MABCY |
49.22 |
47.76 |
-1.46 |
| 9 |
Fulco, Molly (10)
|
MABCY |
51.55 |
47.90 |
-3.65 |
| 10 |
Griffin, Reagan (10)
|
MAPTY |
49.87 |
50.09 |
0.22 |
| 11 |
Fischbach, Emily K (10)
|
MALYM |
1:00.31 |
50.60 |
-9.71 |
| 12 |
Compton, Sophie G (9)
|
MABCY |
55.89 |
50.95 |
-4.94 |
| 13 |
Testa, Annette (10)
|
MAPTY |
50.11 |
51.05 |
0.94 |
| 14 |
Kelly, Erin E (10)
|
MALYM |
48.61 |
51.21 |
2.60 |
| 15 |
Venuti, Talia M (9)
|
MALYM |
56.37 |
58.21 |
1.84 |
| 16 |
Lucas, Elsa (9)
|
MAPTY |
1:07.23 |
58.89 |
-8.34 |
| 17 |
Phillips, Ceri B (9)
|
MARKRY |
57.22 |
1:00.72 |
3.50 |
| 18 |
Podduturi, Aditi (9)
|
HATY |
1:10.86 |
1:16.72 |
5.86 |
|
Richard, Colbie (9)
|
MAPTY |
55.82 |
DQ |
- |
|
Karlson, Katherine (9)
|
HATY |
1:11.00 |
DQ |
- |
|
Weissenburger, Emily G (9)
|
MARKRY |
1:11.08 |
NS |
- |