| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hitchcock, Ella B (12)
|
NCYSST |
30.99 |
31.97 |
0.98 |
| 2 |
Schneider, Taia M (11)
|
SCUN |
32.32 |
32.00 |
-0.32 |
| 3 |
Sherrill, Julia E (11)
|
NCWNCY |
33.29 |
32.29 |
-1.00 |
| 4 |
Utterback, Brittany N (12)
|
SCYSSC |
33.48 |
32.65 |
-0.83 |
| 5 |
Parker, Adelyn G (11)
|
SCAASY |
33.34 |
34.25 |
0.91 |
| 6 |
Ruebusch, Morgan W (11)
|
NCYSST |
32.91 |
34.32 |
1.41 |
| 7 |
Ulmer, Dominique (11)
|
SCCCYH |
35.77 |
34.61 |
-1.16 |
| 8 |
Love, Anna E (12)
|
SCYSSC |
40.40 |
35.70 |
-4.70 |
| 9 |
Goad, Charlotte A (11)
|
SCYSSC |
43.23 |
38.14 |
-5.09 |
| 10 |
Murphy, Ella J (12)
|
SCYSSC |
40.57 |
38.38 |
-2.19 |
| 11 |
Malena, Madison S (11)
|
SCCCYH |
38.48 |
39.11 |
0.63 |
| 12 |
Ashworth, Maarijka E (11)
|
SCYSSC |
41.57 |
40.82 |
-0.75 |
| 13 |
Hubbard, Kayleigh (11)
|
SCCCYH |
40.51 |
41.74 |
1.23 |
| 14 |
Sturgill, Isabella G (12)
|
NCYSST |
42.44 |
42.16 |
-0.28 |
| 15 |
Moser, Ava (11)
|
SCCCYH |
47.48 |
43.10 |
-4.38 |
| 16 |
Kulkarni, Sohini D (12)
|
SCYSSC |
42.28 |
43.52 |
1.24 |
| 17 |
Edwards, Abby C (12)
|
NCWNCY |
43.76 |
44.91 |
1.15 |
| 18 |
Moore, Laney R (12)
|
SCYSSC |
1:00.04 |
44.97 |
-15.07 |
| 19 |
Zhang, Grace H (12)
|
SCYSSC |
47.07 |
45.60 |
-1.47 |
| 20 |
Shevchenko, Adelina E (11)
|
NCWNCY |
|
45.70 |
- |
| 21 |
Ford, Alexa J (11)
|
NCWNCY |
51.45 |
46.08 |
-5.37 |
| 22 |
Gilliam, Kierstin G (11)
|
SCYSSC |
|
46.34 |
- |
| 23 |
Walter, Sophie (12)
|
SCCCYH |
46.23 |
46.47 |
0.24 |
| 24 |
Jones, Olivia G (11)
|
SCYSSC |
51.56 |
51.07 |
-0.49 |
| 25 |
Polchow, Kaitlyn E (11)
|
SCYSSC |
52.93 |
51.27 |
-1.66 |
| 26 |
Smith, Jaclyn P (11)
|
SCYSSC |
58.76 |
56.47 |
-2.29 |
| 27 |
Perez, Synthia R (11)
|
SCYSSC |
|
1:03.42 |
- |
| 28 |
Perez, Savanna F (12)
|
SCYSSC |
|
1:05.41 |
- |
|
Reed, Riley A (11)
|
SCYSSC |
43.02 |
NS |
- |
|
Chambers, Savannah M (12)
|
NCYSST |
|
NS |
- |
|
Pope, Amelia C (12)
|
NCYSST |
45.94 |
NS |
- |