| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hitchcock, Ella B (12)
|
NCYSST |
38.18 |
39.75 |
1.57 |
| 2 |
Ruebusch, Morgan W (11)
|
NCYSST |
40.55 |
40.94 |
0.39 |
| 3 |
Walker, Sadie S (11)
|
SCYSSC |
42.78 |
42.43 |
-0.35 |
| 4 |
Hendley, Morgan K (11)
|
NCWNCY |
44.06 |
44.47 |
0.41 |
| 5 |
Murray, Mackenzie Y (12)
|
SCYSSC |
47.32 |
44.91 |
-2.41 |
| 6 |
Parker, Adelyn G (11)
|
SCAASY |
46.92 |
45.02 |
-1.90 |
| 7 |
Moser, Ava (11)
|
SCCCYH |
48.73 |
45.87 |
-2.86 |
| 8 |
Ashworth, Maarijka E (11)
|
SCYSSC |
49.33 |
46.19 |
-3.14 |
| 9 |
Portele, Molly G (11)
|
SCYSSC |
53.70 |
46.37 |
-7.33 |
| 10 |
Reed, Riley A (11)
|
SCYSSC |
48.08 |
46.94 |
-1.14 |
| 11 |
Stiens, Riley K (11)
|
SCYSSC |
53.97 |
48.66 |
-5.31 |
| 12 |
Dvorak, Izabella R (11)
|
NCWNCY |
49.75 |
48.69 |
-1.06 |
| 13 |
Walter, Sophie (12)
|
SCCCYH |
51.03 |
48.96 |
-2.07 |
| 14 |
Alavandi, Nia S (12)
|
SCYSSC |
1:01.26 |
49.69 |
-11.57 |
| 15 |
Gilliam, Kierstin G (11)
|
SCYSSC |
|
53.02 |
- |
| 16 |
Webb, Stella R (11)
|
NCWNCY |
54.03 |
54.15 |
0.12 |
| 17 |
Miller, Anna L (11)
|
NCWNCY |
51.31 |
54.34 |
3.03 |
| 18 |
Edwards, Abby C (12)
|
NCWNCY |
|
55.75 |
- |
| 19 |
Smith, Jaclyn P (11)
|
SCYSSC |
1:01.16 |
58.06 |
-3.10 |
| 20 |
Perez, Synthia R (11)
|
SCYSSC |
|
1:02.75 |
- |
| 21 |
Polchow, Kaitlyn E (11)
|
SCYSSC |
1:07.73 |
1:05.54 |
-2.19 |
| 22 |
Perez, Savanna F (12)
|
SCYSSC |
|
1:15.90 |
- |
|
Pope, Amelia C (12)
|
NCYSST |
|
DQ |
- |
|
Austin, Thylin A (11)
|
SCYSSC |
40.76 |
NS |
- |
|
Chambers, Savannah M (12)
|
NCYSST |
1:01.84 |
NS |
- |