| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Becker, Megan L (10)
|
NCFAST |
2:47.57 |
2:47.51 |
-0.06 |
| 2 |
Ulmer, Dominique (10)
|
SCCCYH |
3:01.05 |
2:50.50 |
-10.55 |
| 3 |
Do, Claire O (9)
|
SCYSSC |
2:52.72 |
2:51.94 |
-0.78 |
| 4 |
Goddard, Helena J (10)
|
NCGCY |
3:04.98 |
3:00.21 |
-4.77 |
| 5 |
Nordlund, Paige (10)
|
SCGYG |
2:55.26 |
3:00.62 |
5.36 |
| 6 |
Hyder, Lily E (10)
|
SCPCY |
2:59.00 |
3:01.43 |
2.43 |
| 7 |
Ruebusch, Morgan W (10)
|
NCYSST |
3:03.06 |
3:02.22 |
-0.84 |
| 8 |
Wilkinson, Presley B (10)
|
NCTYDE |
3:01.90 |
3:02.48 |
0.58 |
| 9 |
Moore, Mary A (9)
|
NCTYDE |
3:05.71 |
3:04.97 |
-0.74 |
| 10 |
Warren, Sarah J (9)
|
VALY |
3:15.29 |
3:05.24 |
-10.05 |
| 11 |
Byrne, Olivia (10)
|
BYAC |
3:11.19 |
3:07.92 |
-3.27 |
| 12 |
Walker, LaiCie (10)
|
VADYST |
3:09.48 |
3:07.98 |
-1.50 |
| 13 |
Luttrell, Savannah D (10)
|
SCYSSC |
3:02.28 |
3:08.93 |
6.65 |
| 14 |
Cooper, Emma S (9)
|
VALY |
3:12.02 |
3:11.10 |
-0.92 |
| 15 |
Judy, Olivia C (10)
|
VALY |
3:11.67 |
3:17.28 |
5.61 |
|
Liu, Reina R (8)
|
NCGCY |
3:16.54 |
DQ |
- |
|
Yaekoub, Lauren N (10)
|
SCAAYM |
3:08.25 |
DQ |
- |
|
Mahland, Kaelyn M (9)
|
VALY |
3:11.30 |
DQ |
- |