| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
BEMBENISTA, JULIA C (11)
|
NITWST |
45.01 |
43.29 |
-1.72 |
| 2 |
MUTTY, VIVIAN M (11)
|
NIEAST |
45.99 |
46.84 |
0.85 |
| 3 |
DEL REGNO, JAINA R (11)
|
NIRAYS |
47.33 |
48.39 |
1.06 |
| 4 |
LEBARON, JULIA L (11)
|
NIHHSC |
|
48.82 |
- |
| 5 |
HARDY, HALEIGHA G (11)
|
NIPVSC |
48.36 |
49.21 |
0.85 |
| 6 |
WITTMEYER, PAIDEN E (11)
|
NIHHSC |
50.63 |
49.28 |
-1.35 |
| 7 |
GRUSENDORF, ADISON E (11)
|
NIGVST |
50.71 |
50.87 |
0.16 |
| 8 |
SPENCER, TALIA B (11)
|
NIEAST |
55.93 |
51.39 |
-4.54 |
| 9 |
TRUBY, JENNA M (11)
|
NIHHSC |
54.85 |
51.43 |
-3.42 |
| 10 |
TORCHIA, JULIA K (11)
|
NIHAMB |
55.14 |
53.78 |
-1.36 |
| 11 |
JONES, SOPHIA M (11)
|
NIPVSC |
53.51 |
53.90 |
0.39 |
| 12 |
MANCUSO, GABRIELLA J (11)
|
NIHAMB |
54.46 |
54.61 |
0.15 |
| 13 |
HAGGSTROM, RAYGEN M (11)
|
NIGVST |
|
54.86 |
- |
| 14 |
GILCRIST, GRACIE M (11)
|
NISWAT |
58.87 |
55.77 |
-3.10 |
| 15 |
EVANS, NATALIE P (11)
|
NIHAMB |
57.97 |
56.43 |
-1.54 |
| 16 |
WDOWIASZ, GRACIE J (11)
|
NIHHSC |
53.47 |
57.30 |
3.83 |
| 17 |
BAUMAN, CAMERYN (11)
|
NIEAST |
1:02.05 |
58.66 |
-3.39 |
|
SAUER, EMMA L (11)
|
NIHAMB |
43.09 |
41.53 |
-1.56 |
|
WILLIAMS, CIERRA M (11)
|
NITTSC |
45.48 |
DQ |
- |
|
SCHMITT, LARRISSA R (11)
|
NIHAMB |
1:01.91 |
NS |
- |