| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
BARRERA-HAGNER, ANANYA S (10)
|
NIEAST |
45.61 |
44.73 |
-0.88 |
| 2 |
COOK, AMELIA A (10)
|
NIHAMB |
46.35 |
45.02 |
-1.33 |
| 3 |
MCKNIGHT, SARAH (10)
|
NIAMTS |
48.12 |
47.95 |
-0.17 |
| 4 |
IRELAND, ALEXIS M (10)
|
NIGVST |
54.09 |
49.59 |
-4.50 |
| 5 |
CHASE, ABBIGALE B (10)
|
NIFKVL |
49.83 |
50.55 |
0.72 |
| 6 |
SAJDAK, SYDNEY R (10)
|
NIHAMB |
1:00.64 |
1:03.40 |
2.76 |
| 7 |
LEHNEN, ROMANA J (10)
|
NIHHSC |
|
1:06.95 |
- |
| 8 |
KRAJEWSKI, DANIELLE L (10)
|
NIGWST |
|
1:09.53 |
- |
|
FABIANO, MACKENZIE G (10)
|
NIHHSC |
45.67 |
42.62 |
-3.05 |
|
KLEINMANN, ISABELLE K (10)
|
NIHAMB |
45.43 |
42.65 |
-2.78 |
|
WAGNER, AVA I (10)
|
NITWST |
46.98 |
44.63 |
-2.35 |
|
HORN, ERIN E (10)
|
NIEAST |
51.28 |
DQ |
- |
|
LAVERDI, ABIGAIL G (10)
|
NIHAMB |
46.53 |
DQ |
- |
|
HARTUNG, AVA M (10)
|
NIHHSC |
|
NS |
- |