| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
HAGGERTY, GAVIN M (10)
|
NIGVST |
46.33 |
45.78 |
-0.55 |
| 2 |
CLARK, LIAM J (10)
|
NITWST |
44.73 |
46.06 |
1.33 |
| 3 |
STEINER, IAN M (10)
|
NITWST |
45.55 |
46.79 |
1.24 |
| 4 |
SUFFOLETTO, JACOB R (10)
|
NITWST |
50.53 |
48.78 |
-1.75 |
| 5 |
SIONKIEWICZ, WILLIAM D (10)
|
NITTAC |
57.52 |
49.00 |
-8.52 |
| 6 |
CRONE, JAYDEN P (10)
|
NISWAT |
56.53 |
51.48 |
-5.05 |
| 7 |
VITKO, KADEN L (10)
|
NIHAMB |
1:12.39 |
59.03 |
-13.36 |
| 8 |
PHILLIPS, HAYDEN R (10)
|
NIFKVL |
1:01.32 |
1:04.56 |
3.24 |
|
RUDNICKI, WILLIAM S (10)
|
NITWST |
46.43 |
43.51 |
-2.92 |
|
CERASANI, ASA J (10)
|
NISWAT |
44.97 |
44.40 |
-0.57 |
|
SCRANTON, BENJAMIN J (10)
|
NITWST |
1:04.71 |
DQ |
- |