| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
KERINS, BAILEY R (11)
|
NIHAMB |
41.51 |
38.15 |
-3.36 |
| 2 |
MERSINGER, SOPHIA R (11)
|
NITWST |
42.12 |
39.85 |
-2.27 |
| 3 |
MOLDENHAUER, PAIGE A (11)
|
NIEAST |
41.70 |
40.73 |
-0.97 |
| 4 |
HERMAN, TYYETTA R (11)
|
NIFKVL |
42.82 |
42.22 |
-0.60 |
| 5 |
ZIOLKOWSKI, COSETTE M (11)
|
NITTAC |
41.52 |
42.56 |
1.04 |
| 6 |
SPACCIAPOLLI, JULIANNA M (11)
|
NIHHSC |
46.50 |
43.71 |
-2.79 |
| 7 |
RODGERS, MORGAN M (11)
|
NITWST |
50.16 |
45.80 |
-4.36 |
| 8 |
HAAK, ELISE C (11)
|
NIHAMB |
53.13 |
47.33 |
-5.80 |
| 9 |
SAYE, GRACE R (11)
|
NIHHSC |
45.46 |
47.64 |
2.18 |
| 10 |
SHEPPERD, SLOANE A (11)
|
NIEAST |
55.09 |
47.89 |
-7.20 |
| 11 |
MENTLEY-PETERS, WILLOW S (11)
|
NIGWST |
48.67 |
52.25 |
3.58 |