| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
ZOLET, MARTA E (12)
|
NIEAST |
47.35 |
37.07 |
-10.28 |
| 2 |
KUBIAK, KELLY A (12)
|
NITWST |
37.20 |
38.45 |
1.25 |
| 3 |
ROBERTS, ALAINA G (12)
|
NITTSC |
41.90 |
38.46 |
-3.44 |
| 4 |
BUTLER, KENNEDY L (12)
|
NIGVST |
44.60 |
38.99 |
-5.61 |
| 5 |
KOWELL-URE, PHOENIX W (12)
|
NIEAST |
38.75 |
39.11 |
0.36 |
| 6 |
DAHL, SYDNEY R (12)
|
NIPVSC |
43.84 |
39.40 |
-4.44 |
| 7 |
SAINSBURY, MEGHAN A (12)
|
NITWST |
44.33 |
39.91 |
-4.42 |
| 8 |
ASTALOS, HOLLY T (12)
|
NIHAMB |
41.17 |
40.04 |
-1.13 |
| 9 |
KEESLER, KAYLYNN N (12)
|
NIEMAC |
|
40.21 |
- |
| 10 |
CROWE, SAMANTHA G (12)
|
NIHAMB |
43.11 |
42.72 |
-0.39 |
|
HARPER, AMELIA D (12)
|
NIHHSC |
39.53 |
32.61 |
-6.92 |
|
MCCUTCHEON, CHLOE M (12)
|
NIAAAC |
40.92 |
34.54 |
-6.38 |
|
GREENE, EMILY M (12)
|
NIAAAC |
41.65 |
35.49 |
-6.16 |
|
POWERS, AMELIA T (12)
|
NITTAC |
36.34 |
35.56 |
-0.78 |
|
KLOC, CAROLINE C (12)
|
NITWST |
39.28 |
35.80 |
-3.48 |
|
GONZALEZ, CATHERINE E (12)
|
NIHAMB |
45.21 |
DQ |
- |
|
CHAPLIN, ALLISON V (12)
|
NITTSC |
38.18 |
DQ |
- |
|
MARCHESON, MADISON E (12)
|
NIAAAC |
|
NS |
- |