| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
MERSINGER, SOPHIA R (11)
|
NITWST |
40.17 |
38.55 |
-1.62 |
| 2 |
KERINS, BAILEY R (11)
|
NIHAMB |
52.70 |
40.90 |
-11.80 |
| 3 |
MOLDENHAUER, PAIGE A (11)
|
NIEAST |
38.58 |
41.30 |
2.72 |
| 4 |
ZIOLKOWSKI, COSETTE M (11)
|
NITTAC |
39.38 |
41.54 |
2.16 |
| 5 |
MCCORMICK, GRACE A (11)
|
NITWST |
49.72 |
45.25 |
-4.47 |
| 6 |
WILSON, GABRIELLE O (11)
|
NITWST |
47.30 |
47.07 |
-0.23 |
| 7 |
SAND, MAKAYLA E (11)
|
NITWST |
50.13 |
47.65 |
-2.48 |
| 8 |
SPACCIAPOLLI, JULIANNA M (11)
|
NIHHSC |
52.68 |
49.98 |
-2.70 |
| 9 |
SHEPPERD, SLOANE A (11)
|
NIEAST |
57.79 |
54.58 |
-3.21 |
| 10 |
RODGERS, MORGAN M (11)
|
NITWST |
52.38 |
1:01.25 |
8.87 |
| 11 |
CAPOZZI, ALIVIA M (11)
|
NIGWST |
59.58 |
1:04.23 |
4.65 |