| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mathieu, Tajanai S (8)
|
MAPVA |
45.63 |
40.50 |
-5.13 |
| 2 |
Blizzard, Mackenzie E (7)
|
MAUN |
42.69 |
40.89 |
-1.80 |
| 3 |
Fenton, Kirra (8)
|
MAJCCS |
43.66 |
40.96 |
-2.70 |
| 4 |
Grawl, Trina M (8)
|
MABGH |
41.94 |
41.09 |
-0.85 |
| 5 |
Davidson, Aubrey E (7)
|
MAUN |
45.73 |
41.90 |
-3.83 |
| 6 |
Crouthamel, Lauren C (7)
|
MAWWC |
51.59 |
44.17 |
-7.42 |
| 7 |
Holzer, Avery G (6)
|
MAECCC |
58.90 |
45.12 |
-13.78 |
| 8 |
Bird, Avery G (8)
|
MASEA |
50.77 |
47.28 |
-3.49 |
| 9 |
Robbins, Adeline F (7)
|
MAECCC |
49.76 |
47.96 |
-1.80 |
| 10 |
Morris, Eleanor J (8)
|
MASEA |
1:05.93 |
48.36 |
-17.57 |
| 11 |
Sarni, Mia (8)
|
MASEA |
53.82 |
49.97 |
-3.85 |
| 12 |
Otterburn-Martinez, Victoria (8)
|
MASEA |
|
50.96 |
- |
| 13 |
Macalalad, Gianna T (8)
|
MASEA |
|
51.18 |
- |
|
Nordberg, Kaitlyn G (7)
|
MAWWC |
1:00.21 |
DQ |
- |
|
Baron, Isabella M (8)
|
MAUN |
54.70 |
NS |
- |