| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kassirer, Natalie G (12)
|
NITWST |
33.04 |
33.38 |
0.34 |
| 2 |
Walters, Elizabeth J (12)
|
NITWST |
36.70 |
33.39 |
-3.31 |
| 3 |
Canestrari, Willow G (11)
|
NITWST |
38.30 |
38.34 |
0.04 |
| 4 |
Urbanek, Addison E (11)
|
NICLSC |
43.08 |
40.62 |
-2.46 |
| 5 |
Fabiano, Mackenzie G (11)
|
NIHHSC |
40.34 |
41.56 |
1.22 |
| 6 |
Kreher, Laura G (11)
|
NICLSC |
|
42.50 |
- |
| 7 |
Umansky, Isabella C (11)
|
NIBAAC |
40.99 |
43.42 |
2.43 |
| 8 |
Murphy, Audrey L (12)
|
NIEAST |
|
43.49 |
- |
| 9 |
Gavin, Lily L (11)
|
NISWAT |
46.63 |
43.70 |
-2.93 |
| 10 |
Burnett, Ella R (12)
|
NITWST |
1:01.81 |
46.64 |
-15.17 |
| 11 |
Steck, Marina (11)
|
NIAMTS |
47.59 |
48.20 |
0.61 |
| 12 |
Moch, Hope M (11)
|
NITTAC |
46.60 |
49.63 |
3.03 |
| 13 |
Sajdak, Sydney R (11)
|
NIHAMB |
1:00.64 |
50.02 |
-10.62 |
| 14 |
Gascoyne, Louisa M (11)
|
NIEAST |
|
53.03 |
- |
| 15 |
Moch, Faith L (11)
|
NITTAC |
52.94 |
56.90 |
3.96 |
|
Jusiak, Isabella N (12)
|
NIUN-H |
|
DQ |
- |
|
McGill, Rose M (11)
|
NIHAMB |
|
NS |
- |
|
Spencer, Elyse (12)
|
NIAMTS |
34.87 |
NS |
- |