| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Moldenhauer, Paige A (10)
|
NIEAST |
38.27 |
37.33 |
-0.94 |
| 2 |
Spacciapolli, Julianna M (10)
|
NIHHSC |
37.79 |
38.46 |
0.67 |
| 3 |
Carney, Reilly M (10)
|
NIGISC |
44.37 |
41.47 |
-2.90 |
| 4 |
Zaremski, Ella R (10)
|
NIGISC |
45.31 |
41.74 |
-3.57 |
| 5 |
Barrera-Hagner, Ananya S (9)
|
NIEAST |
48.76 |
45.39 |
-3.37 |
| 6 |
Decker, Julie R (10)
|
NIHHSC |
44.77 |
45.44 |
0.67 |
| 7 |
Carney, Reagan D (9)
|
NIGISC |
46.14 |
46.03 |
-0.11 |
| 8 |
Magyari, Eszter (10)
|
NIGISC |
50.32 |
50.50 |
0.18 |
| 9 |
Dugan, Brigid Y (10)
|
NIGISC |
51.20 |
52.78 |
1.58 |
| 10 |
Oldman, Lexi m (9)
|
NIGISC |
|
55.98 |
- |
| 11 |
Nowak, Charlotte K (9)
|
NIGISC |
1:04.56 |
57.36 |
-7.20 |
| 12 |
Winfield, Susannah K (9)
|
NIEAST |
55.70 |
57.66 |
1.96 |
|
Flickinger, Maria J (10)
|
NIEAST |
41.59 |
NS |
- |
|
Horn, Erin E (9)
|
NIEAST |
46.50 |
NS |
- |