| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tedesco, Amanda G (10)
|
MRIA |
34.48 |
33.32 |
-1.16 |
| 2 |
Bendersky, Hailey R (10)
|
MRWISC |
36.03 |
36.57 |
0.54 |
| 3 |
Nguyen, Chloe T (10)
|
MRSSC |
37.23 |
37.65 |
0.42 |
| 4 |
Trobiano, Nicolette P (10)
|
MRFA |
49.99 |
40.78 |
-9.21 |
| 5 |
Petrovic, Branka D (9)
|
MRIA |
40.32 |
42.39 |
2.07 |
| 6 |
Wallace, Evangeline R (9)
|
MRSSC |
44.20 |
42.55 |
-1.65 |
| 7 |
Barron, Emma H (10)
|
MRWISC |
44.95 |
43.37 |
-1.58 |
| 8 |
Jordan, Lily M (10)
|
MRWISC |
40.84 |
43.49 |
2.65 |
| 9 |
Mossey, Rayna M (10)
|
MRTVSC |
42.14 |
43.52 |
1.38 |
| 10 |
Johnson, Ella (10)
|
MRIA |
44.12 |
45.49 |
1.37 |
| 11 |
Cunniffe, Josephine E (10)
|
MRIA |
48.45 |
47.20 |
-1.25 |
| 12 |
McDonald, Karina M (10)
|
MRIA |
51.68 |
49.28 |
-2.40 |
| 13 |
Baynon, Lily M (9)
|
MRFA |
1:12.26 |
49.81 |
-22.45 |
| 14 |
Nguyen, Olivia B (9)
|
MRSSC |
49.93 |
50.31 |
0.38 |
| 15 |
Boyon, Lily A (9)
|
MRFA |
50.01 |
50.41 |
0.40 |
| 16 |
Tian, Maxine (9)
|
MRLIFE |
50.89 |
50.83 |
-0.06 |
| 17 |
Ware, Aurora (8)
|
MRLIFE |
51.30 |
52.95 |
1.65 |
| 18 |
Castano, Gianna I (10)
|
MRFA |
59.99 |
54.31 |
-5.68 |
| 19 |
Casey, Emily C (9)
|
MRTVSC |
55.51 |
55.21 |
-0.30 |
| 20 |
Knopf, Cristina R (9)
|
MRSSC |
57.95 |
57.26 |
-0.69 |
| 21 |
Timm, Avery R (9)
|
MRTVSC |
49.75 |
57.70 |
7.95 |
| 22 |
Caniano, Jenna D (10)
|
MRFA |
1:04.65 |
1:00.15 |
-4.50 |
| 23 |
Tappin, Abigail T (10)
|
MRSSC |
1:04.86 |
1:01.78 |
-3.08 |
| 24 |
McNamara, Kelly L (9)
|
MRSSC |
1:13.94 |
1:06.18 |
-7.76 |
| 25 |
Stanton-Barnett, Baylee M (7)
|
MRSSC |
1:18.77 |
1:17.54 |
-1.23 |
|
Morgan, Maya (7)
|
MRLIFE |
|
NS |
- |
|
Cabrejo, Natali * (10)
|
MRIA |
45.54 |
NS |
- |