| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Moran, Laura A (15)
|
MRQNS |
56.63 |
57.70 |
1.07 |
| 2 |
Chang, Isabella M (15)
|
MRQNS |
56.98 |
57.86 |
0.88 |
| 3 |
Pfisterer, Brooke E (15)
|
MRSSC |
58.78 |
59.64 |
0.86 |
| 4 |
Bieganowski, Maya A (15)
|
MRQNS |
59.32 |
1:00.26 |
0.94 |
| 5 |
Loftus, Ava E (15)
|
MRQNS |
57.82 |
1:02.26 |
4.44 |
| 6 |
Zhang, Erica A (15)
|
MRQNS |
1:05.78 |
1:02.46 |
-3.32 |
| 7 |
Bouzarouata, Salma K (15)
|
MRQNS |
1:02.11 |
1:04.18 |
2.07 |
| 8 |
Marotta, Alexa P (15)
|
MRSSC |
1:06.00 |
1:06.10 |
0.10 |
| 9 |
Selvaggio, Theresa L (16)
|
MRSSC |
1:11.00 |
1:09.87 |
-1.13 |
| 10 |
Smith, Jamila (16)
|
MRNFS |
1:04.03 |
1:11.58 |
7.55 |
| 11 |
Curtin, Olivia M (15)
|
MRSSC |
1:13.61 |
1:15.17 |
1.56 |
| 12 |
LoBello, Allison (16)
|
MRLIFE |
1:35.21 |
1:22.22 |
-12.99 |