| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rollen, Olivia G (17)
|
OKACE |
27.28 |
28.82 |
1.54 |
| 2 |
Rizo, Sofia C (17)
|
NTSNAP |
29.68 |
29.66 |
-0.02 |
| 3 |
White, Amberly C (20)
|
OKAESC |
30.20 |
30.35 |
0.15 |
| 4 |
Catton, Kylie A (17)
|
OKSAC |
28.63 |
30.39 |
1.76 |
| 5 |
Thompson, DeShayla T (17)
|
OKBAC |
30.73 |
30.71 |
-0.02 |
| 6 |
Sims, Laney (17)
|
OKTAC |
31.17 |
31.92 |
0.75 |
| 7 |
Rejman, Olivia A (17)
|
OKACE |
|
33.35 |
- |
| 8 |
Mauldin, Marsha L (74)
|
OKSAC |
52.04 |
49.48 |
-2.56 |
|
Chatzigiannidis, Sophia I (17)
|
OKJTSC |
31.47 |
NS |
- |
|
Bush, Katherine E (17)
|
LARRAC |
28.25 |
NS |
- |
|
Feeney, Charlotte R (17)
|
LARRAC |
37.96 |
NS |
- |