| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hahn, Milla G (9)
|
MAPSC |
38.93 |
37.82 |
-1.11 |
| 1 |
Miller, Amelia E (10)
|
MAGRA |
42.84 |
39.16 |
-3.68 |
| 2 |
Pappentick, Addison K (10)
|
MAEMAC |
44.99 |
41.67 |
-3.32 |
| 3 |
Kushner, Emma M (10)
|
MAPSC |
42.83 |
42.03 |
-0.80 |
| 3 |
Harmon, Sophia D (10)
|
MAEMAC |
44.45 |
42.03 |
-2.42 |
| 5 |
Coombe, Taylor B (10)
|
MAPSC |
46.63 |
42.58 |
-4.05 |
| 6 |
Price, Katryna M (10)
|
MAEMAC |
42.75 |
42.74 |
-0.01 |
| 7 |
Zhang, Grace L (9)
|
MAPSC |
|
43.95 |
- |
| 8 |
Fiore, Autumn M (10)
|
MAUN-0 |
47.59 |
47.41 |
-0.18 |
| 9 |
Platt, Mackenzie M (10)
|
MAPSC |
|
47.48 |
- |
| 10 |
Wagner, Addison B (9)
|
MABLUE |
50.88 |
48.89 |
-1.99 |
| 11 |
Lamm, Ava R (10)
|
MAEMAC |
|
49.52 |
- |
| 12 |
Castro, Emma (10)
|
MABLUE |
52.11 |
50.02 |
-2.09 |
| 13 |
Abramovich, Elizabeth (9)
|
MAEMAC |
|
50.25 |
- |
| 14 |
McPeek, Kelsey M (9)
|
MAPSC |
54.66 |
51.38 |
-3.28 |
| 15 |
Heffner, Kaitlyn S (10)
|
MAEMAC |
|
51.95 |
- |
| 16 |
Castro, Steffi (10)
|
MABLUE |
51.69 |
52.46 |
0.77 |
| 17 |
Newhard, Kate E (9)
|
MAEMAC |
|
52.59 |
- |
| 18 |
Vezenov, Zoe E (9)
|
MAEMAC |
|
53.55 |
- |
| 19 |
Simons, Jillian M (10)
|
MABLUE |
59.18 |
53.70 |
-5.48 |
| 20 |
Skirlis, Samantha T (10)
|
MABLUE |
55.71 |
54.98 |
-0.73 |
| 21 |
Burke, Ella M (10)
|
MABLUE |
54.29 |
55.58 |
1.29 |
| 22 |
Reck, Gianna N (9)
|
MAPSC |
|
55.64 |
- |
| 23 |
Best, Cayla G (9)
|
MAPSC |
|
57.36 |
- |
| 24 |
Goense, Holly P (10)
|
MAPSC |
|
57.65 |
- |
| 25 |
Major, Abigail L (9)
|
MABLUE |
55.60 |
58.73 |
3.13 |
| 26 |
Hock, Sarah J (9)
|
MABLUE |
|
59.43 |
- |
| 27 |
Varga, Delaney (9)
|
MAEMAC |
|
1:00.33 |
- |
| 28 |
Salari, Lillain S (9)
|
MAPAAC |
|
1:01.14 |
- |
| 29 |
Schellenberger, Lilli E (9)
|
MABLUE |
|
1:02.63 |
- |
| 30 |
Quick, Mikayla A (9)
|
MABLUE |
1:09.58 |
1:04.36 |
-5.22 |
|
Reddy, Druthi A (9)
|
MAEMAC |
|
NS |
- |