| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Phelps, Anna E (9)
|
MAUN-0 |
37.25 |
37.35 |
0.10 |
| 2 |
Collins, Ryleigh (9)
|
MAPFY |
39.65 |
41.89 |
2.24 |
| 3 |
Zerfass, Ava D (9)
|
MAEMAC |
46.44 |
45.41 |
-1.03 |
| 4 |
Iacobelli, Sophia C (9)
|
MAEMAC |
43.69 |
45.46 |
1.77 |
| 5 |
Lenett, Carly A (9)
|
MAEMAC |
|
46.01 |
- |
| 6 |
Lin, Simone (9)
|
MAPAAC |
47.65 |
46.61 |
-1.04 |
| 7 |
Frank, Alice C (9)
|
MAEMAC |
48.80 |
48.22 |
-0.58 |
| 8 |
Piacenti, Lily (9)
|
MAPFY |
|
49.37 |
- |
| 9 |
Settle, Ashley (9)
|
MAUNPA |
|
49.59 |
- |
| 10 |
Karnhaukhova, Anastasiya (9)
|
MAPJA |
50.24 |
50.87 |
0.63 |
| 11 |
Wiragh, Kendall J (9)
|
MAEMAC |
49.63 |
52.06 |
2.43 |
| 12 |
Sadler, Cheyenne N (9)
|
MAUNAA |
1:03.40 |
57.91 |
-5.49 |
|
Tassinari, Marianne E (9)
|
MAPFY |
40.37 |
DQ |
- |